Table 1 Details of samples of Sri Lankan Pethia from which sequences were generated, with their localities, voucher references and GenBank accession numbers. LK, Sri Lanka

Pethia melanomaculata

 DZ1500

LK: Ulhitiya, Mahaweli (01)

7.3799 N 81.0939 E

MT732729

M3

MT732764

M1

 DZ1501

LK: Ulhitiya, Mahaweli (01)

7.3799 N 81.0939 E

MZ686566

M3

–

–

 DZ4302

LK: Angammedilla, Mahaweli (02)

7.9140 N 80.9520 E

MZ686567

M3

MZ686661

M1

 DZ4303

LK: Angammedilla, Mahaweli (03)

7.8884 N 80.9116 E

MZ686568

M4

–

–

 DZ4536

LK: Dambulu Oya, Dambulla, Kala (04)

7.8083 N 80.6584 E

MZ686569

M1

MZ686662

M1

 DZ4793

LK: Maha Elagamuwa, Kala (05)

7.9898 N 80.6184 E

MZ686570

M1

MZ686663

M1

 DZ4885

LK: Rajanganaya, Kala (06)

8.1415 N 80.2211 E

MZ686571

M1

MZ686664

M1

 DZ5094

LK: Medawacchiya, Malwathu (07)

8.5091 N 80.4229 E

MZ686572

M1

MZ686665

M1

 DZ5095

LK: Medawacchiya, Malwathu (07)

8.5091 N 80.4229 E

MZ686573

M2

–

–

 DZ4570

LK: Kotagama, Gal (08)

7.1485 N 81.1715 E

MZ686574

M5

MZ686666

M2

 DZ4583

LK: Bellan Oya, Kumbukkan (09)

6.9458 N 81.2874 E

MZ686575

M6

MZ686667

M1

 DZ4584

LK: Bellan Oya, Kumbukkan (09)

6.9458 N 81.2874 E

MZ686576

M7

–

–

Pethia cumingii

 DZ5037

LK: Homadola, Gin (10)

6.2254 N 80.339 E

MZ686577

C9

MZ686668

R1

 DZ5038

LK: Homadola, Gin (10)

6.2254 N 80.339 E

MZ686578

C9

–

–

 DZ5039

LK: Homadola, Gin (10)

6.2254 N 80.339 E

MZ686579

C9

–

–

 DZ4959

LK: Hiniduma, Gin (11)

6.3033 N 80.3193 E

MZ686580

C6

MZ686669

R1

 DZ4960

LK: Hiniduma, Gin (11)

6.3033 N 80.3193 E

MZ686581

C8

MZ686670

R1

 DZ4961

LK: Hiniduma, Gin (11)

6.3033 N 80.3193 E

MZ686582

C9

–

–

 DZ4962

LK: Hiniduma, Gin (11)

6.3033 N 80.3193 E

MZ686583

C5

–

–

 DZ4963

LK: Hiniduma, Gin (11)

6.3033 N 80.3193 E

MZ686584

C7

–

–

 DZ3305

LK: Bambarawana, Mattaka, Bentara (12)

6.3076 N 80.2524 E

MT732731

C4

MT732766

R1

 WHT01

LK: Bambarawana, Mattaka, Bentara (13)

6.3067 N 80.2804 E

MZ686585

C5

MZ686671

R1

 DZ3026

LK: Ratnapura, Elapatha, Kalu (14)

6.6805 N 80.3499 E

MZ686586

C2

MZ686672

R1

 DZ3056

LK: Ratnapura, Elapatha, Kalu (14)

6.6805 N 80.3499 E

MZ686587

C1

MZ686673

R4

 DZ3117

LK: Dombagaskanda, Kalu (15)

6.7245 N 80.1606 E

MZ686588

C3

–

–

 DZ3118

LK: Dombagaskanda, Kalu (15)

6.7245 N 80.1606 E

MZ686589

C2

MZ686674

R3

 DZ3119

LK: Dombagaskanda, Kalu (15)

6.7245 N 80.1606 E

MZ686590

C1

MZ686675

R2

Pethia reval

 WHT03

LK: Yatiyanthota, Kelani (16)

7.0329 N 80.2929 E

MZ686591

R8

MZ686676

R1

 DZ4999

LK: Yogama, Kelani (17)

6.9245 N 80.2746 E

MZ686592

R7

MZ686677

R1

 DZ5000

LK: Yogama, Kelani (17)

6.9245 N 80.2746 E

MZ686593

R8

MZ686678

R1

 DZ5001

LK: Yogama, Kelani (17)

6.9245 N 80.2746 E

MZ686594

R8

–

–

 DZ5002

LK: Yogama, Kelani (17)

6.9245 N 80.2746 E

MZ686595

R8

–

–

 DZ5003

LK: Yogama, Kelani (17)

6.9245 N 80.2746 E

MZ686596

R8

–

–

 DZ3007

LK: Gampaha, Attanagalu (18)

7.0962 N 79.984 E

MZ686597

R5

–

–

 DZ3091

LK: Gampaha, Attanagalu (18)

7.0962 N 79.984 E

MZ686598

R5

–

–

 DZ5032

LK: Yakkala, Attanagalu (19)

7.0792 N 80.0732 E

MZ686599

R5

MZ686679

R1

 DZ5033

LK: Yakkala, Attanagalu (19)

7.0792 N 80.0732 E

MZ686600

R5

MZ686680

R1

 DZ5034

LK: Yakkala, Attanagalu (19)

7.0792 N 80.0732 E

MZ686601

R6

–

–

 DZ5035

LK: Yakkala, Attanagalu (19)

7.0792 N 80.0732 E

MZ686602

C9

–

–

 DZ5036

LK: Yakkala, Attanagalu (19)

7.0792 N 80.0732 E

MZ686603

C9

–

–

 DZ4385

LK: Bopitiya, Giriulla, Ma (20)

7.3121 N 80.0888 E

MZ686604

R3

MZ686681

R1

 DZ4386

LK: Bopitiya, Giriulla, Ma (20)

7.3121 N 80.0888 E

MZ686605

R4

MZ686682

R1

 DZ4828

LK: Bopitiya, Giriulla, Ma (20)

7.3121 N 80.0888 E

MZ686606

R5

MZ686683

R1

 DZ4829

LK: Bopitiya, Giriulla, Ma (20)

7.3121 N 80.0888 E

MZ686607

R3

MZ686684

R1

 DZ4830

LK: Bopitiya, Giriulla, Ma (20)

7.3121 N 80.0888 E

MZ686608

R5

–

–

 DZ3039

LK: Kolamunu Oya, Deduru (21)

7.6029 N 79.9502 E

MZ686609

R2

–

–

 DZ3061

LK: Kolamunu Oya, Deduru (21)

7.6029 N 79.9502 E

MT732730

R1

MT732765

R1

 DZ4823

LK: Nillamba, Hindagala, Mahaweli (22)

7.1861 N 80.6344 E

MZ686610

R11

–

–

 DZ4824

LK: Nillamba, Hindagala, Mahaweli (22)

7.1861 N 80.6344 E

MZ686611

R11

–

–

 DZ4825

LK: Nillamba, Hindagala, Mahaweli (22)

7.1861 N 80.6344 E

MZ686612

R11

–

–

 DZ4826

LK: Nillamba, Hindagala, Mahaweli (22)

7.1861 N 80.6344 E

MZ686613

R11

–

–

 DZ4827

LK: Nillamba, Hindagala, Mahaweli (22)

7.1861 N 80.6344 E

MZ686614

R10

–

–

 DZ3238

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686615

R9

–

–

 DZ3239

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686616

R8

MZ686685

R1

 DZ3240

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686617

R8

MZ686686

R1

 DZ3241

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686618

R8

–

–

 DZ3256

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686619

R9

–

–

 DZ3255

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686620

R9

–

–

 DZ3257

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686621

R8

–

–

 DZ3278

LK: Sarasavi Oya, Peradeniya, Mahaweli (24)

7.2574 N 80.5957 E

MZ686622

R9

MZ686687

R1

Pethia nigrofasciata

 DZ4449

LK: Alawala, Attanagalu (25)

7.12 N 80.1901 E

MZ686623

N1

MZ686688

N1

 DZ4450

LK: Alawala, Attanagalu (25)

7.12 N 80.1901 E

MZ686624

N2

MZ686689

N1

 DZ4451

LK: Alawala, Attanagalu (25)

7.12 N 80.1901 E

MZ686625

N3

–

–

 DZ5040

LK: Alawala, Attanagalu (25)

7.12 N 80.1901 E

MZ686626

N1

MZ686690

N1

 DZ5145

LK: Lenagala, Attanagalu (26)

7.1047 N 80.2177 E

MZ686627

N2

MZ686691

N1

 DZ4343

LK: Illukowita, Thummodara, Kelani (27)

6.8796 N 80.1694 E

MZ686628

N4

MZ686692

N1

 DZ4344

LK: Illukowita, Thummodara, Kelani (27)

6.8796 N 80.1694 E

MZ686629

N5

–

–

 DZ3024

LK: Ratnapura, Elapatha, Kalu (14)

6.6805 N 80.3499 E

MZ686630

N6

–

–

 DZ3025

LK: Ratnapura, Elapatha, Kalu (14)

6.6805 N 80.3499 E

MZ686631

N7

–

–

 DZ3367

LK: Athwelthota, Kalu (28)

6.5382 N 80.2901 E

MZ686632

N9

MZ686693

N1

 DZ4769

LK: Runakanda Forest, Kalu (29)

6.4548 N 80.3263 E

MZ686633

N8

MZ686694

N1

 DZ4770

LK: Runakanda Forest, Kalu (29)

6.4548 N 80.3263 E

MZ686634

N8

–

–

 DZ3095

LK: Thundola, Horawala, Bentara (30)

6.475 N 80.1524 E

MT732732

N11

MT732767

N4

 DZ3096

LK: Thundola, Horawala, Bentara (30)

6.475 N 80.1524 E

MZ686635

N10

MZ686695

N4

 DZ4964

LK: Hiniduma, Gin (11)

6.3033 N 80.3193 E

MZ686636

N12

MZ686696

N2

 DZ4965

LK: Hiniduma, Gin (11)

6.3033 N 80.3193 E

MZ686637

N13

–

–

 DZ4928

LK: Kottawa Forest, Galle, Gin (31)

6.0986 N 80.3146 E

MZ686638

N14

MZ686697

N3

 DZ4929

LK: Kottawa Forest, Galle, Gin (31)

6.0986 N 80.3146 E

MZ686639

N14

–

–

 DZ4930

LK: Kottawa Forest, Galle, Gin (31)

6.0986 N 80.3146 E

MZ686640

N15

–

–

 DZ4478

LK: Ugudu dola, Opatha, Nilwala (32)

6.2669 N 80.4023 E

MZ686641

N17

MZ686698

N2

 DZ4479

LK: Ugudu dola, Opatha, Nilwala (32)

6.2669 N 80.4023 E

MZ686642

N18

MZ686699

N2

 DZ5060

LK: Kotapola, Nilwala (33)

6.2949 N 80.544 E

MZ686643

N16

MZ686700

N1

 DZ5061

LK: Kotapola, Nilwala (33)

6.2949 N 80.544 E

MZ686644

N16

MZ686701

N1

 DZ5062

LK: Kotapola, Nilwala (33)

6.2949 N 80.544 E

MZ686645

N16

–

–

 DZ1454

LK: Pambahinna, Walawe (34)

6.7018 N 80.8006 E

MZ686646

N19

–

–

 DZ4244

LK: Rajawaka, near Kaltota, Walawe (35)

6.6064 N 80.8195 E

MZ686647

N19

MZ686702

N2

 DZ4245

LK: Rajawaka, near Kaltota, Walawe (35)

6.6064 N 80.8195 E

MZ686648

N19

MZ686703

N1

 DZ3233

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686649

N6

MZ686704

N1

 DZ3234

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686650

N23

–

–

 DZ3236

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686651

N22

–

–

 DZ3237

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686652

N5

–

–

 DZ3263

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686653

N5

–

–

 DZ3264

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686654

N23

–

–

 DZ3265

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686655

N20

–

–

 DZ3266

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686656

N6

–

–

 DZ3267

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686657

N21

–

–

 DZ3268

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686658

N23

–

–

 DZ3269

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686659

N20

–

–

 DZ3270

LK: Ambagamuwa, Mahaweli (23)

7.0151 N 80.4915 E

MZ686660

N20

–

–