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Table 3 Percentages of Bufo bufo individuals that contained various bufadienolide compounds in the two development stages. We could unambiguously identify three compounds based on the standards and detected another 19 compounds as unknown bufadienolides based on their characteristic UV spectra. Analytical properties of the detected compounds are provided. (N: sample size (number of individuals), m/z: mass/charge)

From: Exposure to Batrachochytrium dendrobatidis affects chemical defences in two anuran amphibians, Rana dalmatina and Bufo bufo

Bufadienolide compounds Percentage of individuals containing the compound Analytical properties
Tadpoles (N = 53)
(%)
Toadlets (N = 38)
(%)
Retention time (minute) m/z (M+H+)
Arenobufagin 59 74 4.8 417
Telocinobufagin 34 100 8.8 403
Bufotalin 66 100 9.8 445
Compound 1 9 53 4.1 615
Compound 2 17 100 4.7 699
Compound 3 81 100 5.0 417
Compound 4 26 95 5.8 713
Compound 5 0 58 6.0 601
Compound 6 51 100 6.1 415
Compound 7 100 100 7.0 729
Compound 8 49 100 7.5 701
Compound 9 100 100 8.0 727
Compound 10 0 100 9.3 715
Compound 11 0 100 10.6 713
Compound 12 19 100 11.1 401
Compound 13 96 95 11.3 715
Compound 14 0 100 14.9 701
compound 15 100 95 17.5 757
Compound 16 98 84 19.3 573
Compound 17 100 100 20.5 571
Compound 18 100 97 22.1 367
Compound 19 100 100 23.3 365