Table 3 Percentages of Bufo bufo individuals that contained various bufadienolide compounds in the two development stages. We could unambiguously identify three compounds based on the standards and detected another 19 compounds as unknown bufadienolides based on their characteristic UV spectra. Analytical properties of the detected compounds are provided. (N: sample size (number of individuals), m/z: mass/charge)
Bufadienolide compounds | Percentage of individuals containing the compound | Analytical properties | ||
|---|---|---|---|---|
Tadpoles (N = 53) (%) | Toadlets (N = 38) (%) | Retention time (minute) | m/z (M+H+) | |
Arenobufagin | 59 | 74 | 4.8 | 417 |
Telocinobufagin | 34 | 100 | 8.8 | 403 |
Bufotalin | 66 | 100 | 9.8 | 445 |
Compound 1 | 9 | 53 | 4.1 | 615 |
Compound 2 | 17 | 100 | 4.7 | 699 |
Compound 3 | 81 | 100 | 5.0 | 417 |
Compound 4 | 26 | 95 | 5.8 | 713 |
Compound 5 | 0 | 58 | 6.0 | 601 |
Compound 6 | 51 | 100 | 6.1 | 415 |
Compound 7 | 100 | 100 | 7.0 | 729 |
Compound 8 | 49 | 100 | 7.5 | 701 |
Compound 9 | 100 | 100 | 8.0 | 727 |
Compound 10 | 0 | 100 | 9.3 | 715 |
Compound 11 | 0 | 100 | 10.6 | 713 |
Compound 12 | 19 | 100 | 11.1 | 401 |
Compound 13 | 96 | 95 | 11.3 | 715 |
Compound 14 | 0 | 100 | 14.9 | 701 |
compound 15 | 100 | 95 | 17.5 | 757 |
Compound 16 | 98 | 84 | 19.3 | 573 |
Compound 17 | 100 | 100 | 20.5 | 571 |
Compound 18 | 100 | 97 | 22.1 | 367 |
Compound 19 | 100 | 100 | 23.3 | 365 |