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Fig. 6 | BMC Ecology and Evolution

Fig. 6

From: Evolution of mechanisms controlling epithelial morphogenesis across animals: new insights from dissociation-reaggregation experiments in the sponge Oscarella lobularis

Fig. 6

Characteristics of the de novo transcriptome assembly of O. lobularis. A Contig length distribution and GC content of the O. lobularis transcriptome from mixed stages and types of sequencing. Statistics was done after de novo assembly, concatenation, decontamination, and length cleaning steps. The smallest contig is 255 bp long and the longest contig has 47 628 bp coming from a single long ORF encoding a Mucin ortholog. The average contig length is 958 bp. The mean percentage of GC content is 48.2%. B Annotated coding regions either with eggNOG [65] (orthologs) (18,129 sequences), GO Terms (11,526), and Conserved Domains (CDs) [93] (28,440 sequences) (Additional file 5: Table S2). For 1793 contigs no described CD or ortholog could be found, corresponding to 5.5% of the identified coding regions. C The ten most frequent Conserved Domains (CDs) in the O. lobularis transcriptome. Among the top ten CDs are kinases, nucleotide binding domains, domains that are involved in membrane transport or membrane binding as well as those inferring changes in cell shape. The protein kinase domain was most abundant and was present in 4057 coding regions. Occurrences were counted once per coding region. D Percentage of orthologs, O. lobularis has in common with other species. 29% orthologs are shared with human, 25.2% with Trichoplax adhaerens. Shared orthologs with other sponges vary from 24 to 57%. 56.6% of contigs from O. lobularis have a clear ortholog in its close relative, O. pearsei (Additional file 6: Table S3). E DOGMA analysis [29] to evaluate the completeness of different metazoan transcriptomes (Additional file 8: Table S5). For O. lobularis, we found 72.2% of expected CD arrangements. This is within the range of other sponge transcriptomes

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