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Fig. 4 | BMC Ecology and Evolution

Fig. 4

From: Evolution of mechanisms controlling epithelial morphogenesis across animals: new insights from dissociation-reaggregation experiments in the sponge Oscarella lobularis

Fig. 4

Restoration of the choanoderm during reaggregation. Confocal microscopic views of (A) a choanocyte chamber after 3 h of reaggregation (3hR) in natural sea water (NSW), the immunostaining of type IV collagen (in magenta) is discontinuous (dotted pink arrow). B Focus on three choanocytes starting to realign with each other at 3hR (white arrows). C A choanocyte chamber fully restructured after 24 h of reaggregation (24hR), choanocytes present typical apical microvilli (cyan arrow). D Focus on choanocytes with a restored apical microvilli (cyan arrow). For all pictures, staining in grey: Phalloidin, in Green: Pha-E lectin, in Blue: DAPI, in Magenta: type IV collagen. Additional views of control conditions in NSW are available in Additional file 2: Figures S2B

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BMC Ecology and Evolution

ISSN: 2730-7182

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