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Table 1 Attributes of ISSR primers of Zingiber zerumbet used in the present study

From: Genetic variation and population structure of clonal Zingiber zerumbet at a fine geographic scale: a comparison with two closely related selfing and outcrossing Zingiber species

Primer

Sequence 5´ to 3´

Tm (°C)

SR (bp)

NT

NP

810

(GA)8 T

44

220–2350

22

13

811

(GA)8 T

52

230–2050

22

20

817

(CA)8A

56

270–1750

21

20

826

(AC)8C

56

230–1800

27

21

834

(AG)8Y*T

46

320–2100

23

23

841

(GA)8Y*C

56

180–1850

24

24

847

(CA)8R*C

59

180–2200

38

35

857

(AC)8Y*G

48.5

180–1950

24

19

884

HBH*(AG)7

52.5

220–1850

23

20

887

DVD*(TC)7

52

370–1950

22

20

888

BDB*(CA)7

56

270–2050

21

13

889

DBD*(AC)7

59

270–1850

26

22

Total

–

–

180–2350

293

250

  1. *B = (C, G, T), D = (A, G, T), R = (A, T), V = (A, C, G), Y = (C, G), H = (A, C, T)
  2. Tm annealing temperature, SR size range of amplified fragments, NT total number of bands, NP number of polymorphic bands