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Table 1 Attributes of ISSR primers of Zingiber zerumbet used in the present study

From: Genetic variation and population structure of clonal Zingiber zerumbet at a fine geographic scale: a comparison with two closely related selfing and outcrossing Zingiber species

Primer Sequence 5´ to 3´ Tm (°C) SR (bp) NT NP
810 (GA)8 T 44 220–2350 22 13
811 (GA)8 T 52 230–2050 22 20
817 (CA)8A 56 270–1750 21 20
826 (AC)8C 56 230–1800 27 21
834 (AG)8Y*T 46 320–2100 23 23
841 (GA)8Y*C 56 180–1850 24 24
847 (CA)8R*C 59 180–2200 38 35
857 (AC)8Y*G 48.5 180–1950 24 19
884 HBH*(AG)7 52.5 220–1850 23 20
887 DVD*(TC)7 52 370–1950 22 20
888 BDB*(CA)7 56 270–2050 21 13
889 DBD*(AC)7 59 270–1850 26 22
Total 180–2350 293 250
  1. *B = (C, G, T), D = (A, G, T), R = (A, T), V = (A, C, G), Y = (C, G), H = (A, C, T)
  2. Tm annealing temperature, SR size range of amplified fragments, NT total number of bands, NP number of polymorphic bands