18S rRNA variability maps reveal three highly divergent, conserved motifs within Rotifera

Table 5 Summary statistics regarding indels in each of the three exemplar rotifer 18S rRNA molecules from the complete Rotifera alignment (i.e., excluding Calicophoron calicophorum)

Region	Start position in the aligned rotifer data set	Length in the aligned rotifer data set	Adineta vaga (Bdelloidea)			Brachionus plicatilis (Monogononta)			Seison nebaliae (Seisonacea)
Region	Start position in the aligned rotifer data set	Length in the aligned rotifer data set	Number of nucleotides	Number of indels	Average length of indel	Number of nucleotides	Number of indels	Average length of indel	Number of nucleotides	Number of indels	Average length of indel
V1	64	39	33	4	1.5	33	3	2.0	35	3	1.3
V2	187	125	104	10	2.1	102	10	2.3	104	6	3.5
V3	524	83	74	7	1.3	75	6	1.3	77	4	1.5
V4	700	286	228	27	2.1	233	23	2.3	236	30	1.7
V5	1174	50	44	6	1.0	44	5	1.2	41	7	1.7
V6	1374	42	42	0	n/a	42	0	n/a	42	0	n/a
V7	1491	79	51	3	9.3	48	4	7.8	64	4	3.8
V8	1660	73	60	7	1.9	60	6	2.2	68	4	1.3
V9	1880	54	48	4	1.5	46	4	2.0	34	8	2.5
Non	n/a	1190	1135	50	1.1	1130	52	1.2	1129	51	1.2

For the pooled, non-hypervariable regions (“non”), terminal gaps, which likely represent incomplete sequences, were excluded from the calculations

ISSN: 2730-7182