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Table 4 Putative compensatory mutations in evolved cog7Δ msh2Δ and nup133Δ msh2Δ mutator populations

From: Genetic interaction network has a very limited impact on the evolutionary trajectories in continuous culture-grown populations of yeast

Primarily deleted gene Mutated gene Function aa change aa position PFAM Domain (aa) Motif
(ELM prediction)
Putative connection with deleted gene
COG7 PMR1 Ion transporting ATPase F → C 926 PF00689
P-type ATPase, transmembrane domain (762–934)
  Required for Ca2+ and Mn2+ transport into Golgi
TRX2 Cytoplasmic thioredoxin M → V 40 PF00085 Thioredoxin domain (10–100)   Required for ER vesicle fusion with the Golgi
NUP133 ACC1 Regulates histone acetylation; required for synthesis of long-chain fatty acids that were proposed to stabilize the NPC [41] H → R 2068 PF01039
Acetyl-CoA carboxylase (1574–2130)
  Nup133 also plays a role in transcription regulation and chromatin silencing
BRE1 Required for methylation of selected histones [42] L → S 599   
RSC1 Regulates nucleosome positioning and transcription regulation [43] A → V 98 PF00439
Bromodomain (31–98)
 
SWI1 Regulates transcription by remodeling chromatin [44] T → N 15   GSK3 phosphorylation recognition site
CEX1 Component of nuclear tRNA export pathway [48] A → T 468    Associates with NPCs by interacting with Nup116p
  1. Putative compensatory mutations in evolved cog7Δ msh2Δ and nup133Δ msh2Δ mutator populations