Fig. 3

Schematic description of approach 1, which identified candidate genes based on high relative levels of testicular expression. Of the 546 mouse genes that were highly expressed in the testis, 454 fruit fly orthologs were found using the BioMart tool in Ensembl Metazoa (“Phylogenetic filter”). We applied three filters (filter 1: mutant phenotypes; filter 2: relative levels of testicular expression; filter 3: absolute levels of testicular expression for genes identified via filter 2) to the information in FlyBase to identify reproductive genes in Drosophila. Then, orthologs in hymenopterans were identified using the BioMart tool in Ensembl Metazoa and the various sets of fruit fly genes: the initial set of 454 genes and the subsets of 25 (filter 1), 108 (filter 2), and 71 genes (filters 2 + 3). The numbers are in bold in the text. The mouse data are in red, the fruit fly data are in black, and the hymenopteran data are in green. Drawing of organisms © Eric Imbert/IRBI CNRS/Université de Tours