Fig. 4

Sequence alignment and phylogeny of PR-1i proteins in Moniliophthora isolates. Only a slice of the middle portion of the alignment is shown to highlight the sites with positive selection signs, indicated by red (p-value ≤ 0.01) or orange (p-value ≤ 0.05) bars in the bar chart of omega values below the alignment. Below the bar chart, annotations indicate the locations along the sequence of the CAP domains, caveolin-binding motif (CBM) and alpha-helices (α). On the 3D crystal structure of MpPR-1i protein (PBD:5V50) and on the bar chart, “A” indicates the site under positive selection detected in the CBM and “B” indicates the site under positive selection in alpha-helix 1. For figure of the whole sequence alignment and omega value bar chart of PR-1i and the other PR-1 families, see Additional file 5