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Fig. 1 | BMC Ecology and Evolution

Fig. 1

From: Water immersion tolerance by larval instars of stable fly, Stomoxys calcitrans, L1758 (Diptera: Muscidae) impairs the fitness performance of their subsequent stages

Fig. 1

Overview of experimental methods. a Water immersion tolerance experiment: (1) L1, L2 and L3 larval instars of S. calcitrans reared on rabbit dung, (2) 30 L1, L2 and L3 larvae submerged individually in distilled water, (3) time recorded for L1, L2 and L3 individuals to enter into stress-induced immobility, (4) knocked-out larvae transferred to a paper towel, under ambient condition, (5) time recorded for L1, L2 and L3 individuals to recover from the immobility after being returned to the ambient temperature. b Fitness costs associated with water immersion: (1) L1, L2 and L3 larval instars of S. calcitrans reared on rabbit dung, (2) L1, L2 and L3 larvae transferred in distilled water to initiate immobility, (3) stressed larvae returned to rabbit dung, (4) life-history parameters recorded in stressed larvae after they recovered from the immobility. The clipart depicted in Fig. 1 is the product of my conception

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BMC Ecology and Evolution

ISSN: 2730-7182

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