Fig. 1

Predictions of N-terminal targeting sequences for chloroplast (chHCF101) and mitochondrial versions of HCF101 (mHCF101) in selected alveolates and stramenopiles. Red color highlights the mitochondrial leader sequence with cleavage sites predicted with Mitofates (star), TargetP 2 (circle), and PSORT II (triangle). Yellow color indicates a signal peptide, which is cleaved right before the phenylalanine residue, a typical feature for signal peptide cleavage in diatoms. This cleavage site was predicted with high support by the TargetP, Signal P, and HECTAR algorithms. Green represents a predicted transit peptide with two possible cleavage sites for stromal processing peptidase, manually predicted based on previous studies [92]. Gray color highlights conserved residues