Delimitation of five astome ciliate species isolated from the digestive tube of three ecologically different groups of lumbricid earthworms, using the internal transcribed spacer region and the hypervariable D1/D2 region of the 28S rRNA gene

Table 1 Characterization of the ITS region and partial 28S rRNA gene sequences newly obtained from astome ciliates isolated from lumbricid oligochaetes

Species	Specimen^a	Host species	Locality code^b	Length (nt)	GC (%)	GenBank entry
Anoplophrya lumbrici	KR 11 LT	L. terrestris	KR	1339	48.24	MN897871
Anoplophrya lumbrici	RZ 6 LT	L. terrestris	RZ	1339	48.24	MN897872
Subanoplophrya nodulata	PU 29 OT	O. tyrtaeum	PU	1330	41.73	MN897873
Subanoplophrya nodulata	PU 30 OT	O. tyrtaeum	PU	1330	41.80	MN897874
Anoplophrya vulgaris	JA-1 20 EF	E. fetida	JA-1	1326	51.06	MN897875
	JA-1 21 EF	E. fetida	JA-1	1326	51.06	MN897876
	NG 27 DV	D. veneta	NG	1326	51.06	MN897877
	NG 28 DV	D. veneta	NG	1326	51.06	MN897878
	BZ 13 EF	E. fetida	BZ	1326	51.06	MN897879
Metaradiophrya lumbrici	JA-2 25 LT	L. terrestris	JA-2	1341	46.83	MN897880
	JA-2 26 LT	L. terrestris	JA-2	1341	46.83	MN897881
	KR 8 LT	L. terrestris	KR	1341	46.83	MN897882
	KR 10/1 LT	L. terrestris	KR	1341	46.83	MN897883
	RZ 4 LT	L. terrestris	RZ	1341	46.83	MN897884
	RZ 5 LT	L. terrestris	RZ	1341	46.83	MN897885
Metaradiophrya varians	BZ 12 EF	E. fetida	BZ	1343	46.02	MN897886
	BZ 14 EF	E. fetida	BZ	1343	46.02	MN897887
	JA-1 19 EF	E. fetida	JA-1	1343	46.02	MN897888
	JA-1 22 EF	E. fetida	JA-1	1343	46.02	MN897889

^a Specimen code consists of a locality code as specified in Additional file 1: Table S1, an isolate code, and an abbreviation of host species name specified as follows: DV: Dendrobaena veneta; EF: Eisenia fetida; LT: Lumbricus terrestris; and OT: Octolasion tyrtaeum
^b For locality codes and further details, see Additional file 1: Table S1

ISSN: 2730-7182