Fig. 6
Ca2+ changes in fluorescence intensity of 20 single HEK293T cells upon taste substances. HEK293T cells were stimulated with 200 mM glucose (a), 200 mM fructose (b), and 100 mM arginine (c). Images were recorded at 6.54 s intervals up to 183.16 s using 488 nm excitation filter and 516 nm emission filter and analyzed using FV10-ASW 3.1 Viewer software. The backgrounds of the emission intensities were subtracted. Data are expressed as the ratio of the fluorescence intensities of 20 single HEK293T cells per dish and initial intensity (F/F0)