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Fig. 2 | BMC Evolutionary Biology

Fig. 2

From: Maladaptive plasticity facilitates evolution of thermal tolerance during an experimental range shift

Fig. 2

Experimental design of the experiment. Evolved lines (fluctuating or constant thermal decline over 6 generations; n = 5 lines per treatment) were split into one of two developmental treatments, in which individuals from each replicate line were reared under their evolved conditions, or were reverted back to their ancestral conditions (27 °C) (E). Individuals from lines which were maintained in their evolved conditions for the developmental treatment were then exposed to either a short-term acclimation treatment of 17 °C, representing the lower limits of their treatments (E + D + A), or were acclimated under ancestral conditions (27 °C) (E + D) prior to the start of thermal trials. A constant treatment (also with n = 5 lines) was maintained at 27 °C throughout. Each of the evolved and developmental treatments is represented by n = 5 replicate lines. The acclimation treatments are represented by n = 40 individuals total per developmental treatment category

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