Table 1 Repeated measures ANOVAs of the effects of starting frequency (rare or common) and environmental conditions (homogenous or heterogeneous) on changes in the frequency of mtDNA haplotype HI and the strength of frequency dependent selection in the experimental evolution lines. Generation represents the within-line effect of generation 0–5 versus 5–10
Frequency change of HI (Δf) | Frequency dependent selection coefficient (SI) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Between-line effects: | df | MS | F | P | Pperma | df | MS | F | P | Pperma |
Starting frequency (SF) | 1 | 1.1E-02 | 8.091 | 0.022 | 0.021 | 1 | 2.1E-02 | 0.048 | 0.832 | 0.849 |
Environment (E) | 1 | 1.5E-03 | 1.123 | 0.320 | 0.336 | 1 | 4.5E-02 | 0.104 | 0.756 | 0.786 |
SF × E | 1 | 1.8E-03 | 1.402 | 0.270 | 0.276 | 1 | 2.3E+ 00 | 5.394 | 0.049 | 0.034 |
Residual | 8 | 1.3E-03 | 8 | 4.3E-01 | ||||||
Within-line effects: | ||||||||||
Generation (G) | 1 | 4.5E-06 | 0.001 | 0.972 | 1 | 2.1E-01 | 0.308 | 0.594 | ||
G × SF | 1 | 2.4E-06 | 0.001 | 0.979 | 1 | 4.0E-01 | 0.585 | 0.466 | ||
G × E | 1 | 1.4E-04 | 0.043 | 0.842 | 1 | 1.5E-01 | 0.214 | 0.656 | ||
G × SF × E | 1 | 4.9E-04 | 0.146 | 0.713 | 1 | 1.6E+ 00 | 2.348 | 0.164 | ||
Residual | 8 | 3.4E-03 | 8 | 6.8E-01 | ||||||