Table 1 The marker set for Megachile sculpturalis including primer sequence (F = forward and R = reverse), repetition motif, four primer mixes (PM1—4), amplification length (ASR), polymorphic information content (PIC) and unbiased expected Heterozygosity (uH_eL) per locus

FB1

F: TTTTTCGTCGTCACGCTG

CCTTT-9

PM 1

412–422

0.487

3

0.564

R: CGGAGTTAAAGGAAGGGAAA

FB2

F: ACCGACGCTATTAAAATTGTC

AGAAG-19

PM 3

407–437

0.558

3

0.638

R: TCCACGAAAGAGTTCTTTGT

FB3

F: GTGAAATCGACGCGAAAC

TTTCG-10

PM 4

421–441

0.501

3

0.592

R: ATTGGTCTTCTGGTTCGTTT

FB4

F: GTAGACACCTCGTGACTTTT

GAGAG-9

PM 3

412–422

0.278

3

0.312

R: ATAAGCCGCGACACAAATT

FB5

F: CTGTCGAGTCGGACCTTT

TCTTT-12

PM 1

442–447

0.322

2

0.406

R: ATCGACGAGTTTTTGTACGA

FB6

F: ACGTTCTTCTTACGATTCTTC

ATAGA-8

PM 3

463–478

0.704

5

0.754

R: GGAATGCCTTTTCGAGTTTC

FB7

F: AAGAGAACAATAAGTCGAACG

TCTTT-7

PM 4

360–400

0.566

4

0.643

R: TGAACGTACGTAATTTGCAT

FB8

F: TCGTACGAGAAACGAAAGG

TTTCG-10

PM 3

390–410

0.492

3

0.585

R: AGTCAGACGAGTAGTTAGGC

FB9

F: ACCCCACGAATGTTAACG

CGACG-8

PM 1

398–433

0.519

5

0.573

R: TTTACGGCCGAGTTTTCC

FB10*

F: CGTCAAAGGCTACCGTATAA

AACAG-14

PM 3

436–441

0.2

2

0.227

R: CCTTCAATGTTCAAGGTGAA

FB11

F: CCACTTTATCCGTTTGTTCG

AATCG-9

PM 3

415–430

0.603

4

0.658

R: ACGGCGAAATCGAGTCTTA

FB13

F: TTGAATCTCTGCTCTGTGAC

TTTTC-11

PM 3

442–467

0.576

3

0.656

R: GCGGTGATATTAGACTCGTA

FB14

F: GTCGAGCGATTGGAGTAATC

TACTC-8

PM 1

420–495

0.509

4

0.554

R: CGTACCTCGAAAATACGCT

FB15

F: GAACAGAAGGTTTGTTCGC

TCGTA-9

PM 4

440–485

0.43

5

0.477

R: TTCCATCTGGCACGATAG

FB16

F: CTGAAGTCATTCGGGTAAGA

CGAAA-9

PM 3

412–427

0.478

4

0.538

R: TCGTACTTTTCACCCTGAAA

FB17

F: CGGGCATGAACGAATATTCT

TTCGT-7

PM 1

420–455

0.491

4

0.543

R: TACTTTTAACGTCGCGTATT

FB18*

F: GAACGTTGACCAAGTGGATT

AAAGA-8

PM 3

400–400

0.229

2

0.266

R: AGTTCGAGCTGTCACTTTTT

FB19

F: CTAATTGCCATCGAGCCAG

CGTTC-8

PM 3

445–470

0.501

5

0.557

R: GACGATCTTGGTTAAAACAGT

FB20*

F: CTTCTCGTTCGAGGATCATT

CTTC-8

PM 1

435–435

0

1

0

R: GGTCAAGGTAAAGGAAGGAG

FB21

F: AAGCATCGTACCTCGGTATA

ACGA-5_GAAC-11

PM 1

378–410

0.678

5

0.73

R: GTGTTCCCTTTAAAACTCGC

FB22

F: CTCTGTCTCAGGTTAGTGTG

TCTT-13

PM 3

412–424

0.294

5

0.311

R: AATAGAGCGCATTACCGATT

FB23

F: GATATCGATCCCATCCGAAA

GTGC-12

PM 3

384–400

0.735

7

0.774

R: TCTCACGATGATTATACGTCC

FB24

F: CAAACTTTCCTGGTACCGG

TTTC-11

PM 4

390–402

0.538

3

0.618

R: ACGATTAAATCATTTCGGTTGA

FB25

F: TTGAAATTCAACGTATGCGC

AGAA-11

PM 1

434–434

0.556

5

0.635

R: GAACGGCGAAACTTCTATTC

FB26

F: AATCGAAAAAGAAACACGGG

GAAG-7

PM 3

429–433

0.581

3

0.661

R: AGAAACTCACCTTGTCCATC

FB27

F: ATTGTTGAGGCGAATAACCT

ATGT-10

PM 4

403–443

0.677

5

0.728

R: TCGTTAAGAATGAATGAACGA

FB28

F: CCTTGGTCTCGTCGTTATTA

TCTT-8

PM 3

416–422

0.699

5

0.749

R: GTCGTTTAACCGAGACGC

FB29

F: TACCTTTCGTCAAAGATGCA

TATT-8

PM 3

432–444

0.425

4

0.536

R: ATAGAAGCATGTCAACACCC

FB30

F: CGGGTACGACAAGGATTAAA

CCAA-9

PM 4

427–435

0.592

3

0.671

R: ATCAGCCTAGAGTGTAGAGG

FB31

F: GATCACCTACGTAATGCTGT

GAAC-7

PM 1

421–421

0.535

4

0.611

R: CGCCACTCGCATAAAATTAA

FB32

F: TCCCGGGCAAAGATAAATAT

GTTC-11_TTCG-5

PM 3

409–441

0.676

5

0.728

R: ATATATCGCCTCGTGTTACG

FB33

F: GAAGAGCCTATAGACCCTGT

CCTT-10

PM 3

400–420

0.621

5

0.692

R: AACTATTGCCGAGTTATCCG

FB34*

F: TTTCATAATTTACGCGTCTCTC

ATCT-8

PM 1

400–400

0

1

0

R: ATATCGAATCTACCTCTGTGC

FB35

F: TTAAACTCCATTACGCGTCA

ATAG-12

PM 3

445–453

0.407

3

0.493

R: CATCGCGGACAATAAACTTT

FB36

F: AACTGATCCCCTGCCATT

GCGT-9

PM 4

399–415

0.66

4

0.717

R: TGTTCTAGCGCATCTGATTC

FB37

F: AGCAAAAACGTGAAGAATGA

GCGT-12

PM 3

373–401

0.58

3

0.659

R: AAATTTTCGACCACATACGC

FB38

F: ACCTTAGTCGTTAGTAGCCT

TCTG-7

PM 3

417–421

0.537

3

0.621

R: CGGAAGGAAATTTCGTACGA

FB39

F: GCTGACTTGCACACAAATTT

AAGG-10_AAAG-5

PM 3

406–426

0.66

5

0.715

R: CGATCCCGTTATCTTCGATT

FB40

F: GCAAGAAACAAAAACCGTTG

CTCA-18

PM 1

385–421

0.522

4

0.6

R: CGTTAGTCTGACAGTTTTCG

FB41

F: TTTGCTACAAAACACTGGAC

AGAA-7

PM 3

430–450

0.716

5

0.763

R: GTACCTCTCGAACTTCCTTT

FB42

F: TTGTGTCACCTTTAATCGGT

TTGC-8

PM 4

444–508

0.563

6

0.604

R: GGATAAAAATCCGTGCGAAA

FB43

F: TGTTCGCCTCTAGATCGATA

TCCT-11

PM 3

419–443

0.624

5

0.69

R: GCGTTAATCAAATGGTTCGA

FB44

F: ACGCGAATAATTTACGATGAC

CGTT-10

PM 3

399–407

0.522

4

0.572

R: GATTAAACAAAAGCGGCAAC

FB45

F: GAATCGCCAAAAGTGCATAA

GAAG-14

PM 1

438–454

0.376

3

0.491

R: TACTTTCGAGTCTACTTGCC

FB46

F: CCGGGGGAAAGTTCAATTTA

GAAA-9_AGAA-11

PM 4

435–507

0.525

6

0.593

R: TTGTAATTTGCAATCGCGAT

FB48

F: GAAAAGGAAAGTCGAAACGG

CGTT-9

PM 4

373–405

0.557

4

0.634

R: TACCAGATAGAAACGCGATG