DNA barcoding reveals cryptic diversity in the underestimated genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the northeastern Qinghai-Tibet Plateau

Table 2 Genetic K2P distances of the Triplophysa species

Species	MOTU	Mean intra-	Maximum intra-	NN Dist	NN
Triplophysa bleekeri	MOTU-1	0.0000	0.0000	0.0731	T. papillosolabiatus
T. shiyangensis	MOTU-3	0.0069	0.0130	0.0265	T. stoliczkae
T. strauchii	MOTU-4	0.0017	0.0020	0.0271	T. stoliczkae
T. orientalis	MOTU-5	0.0000	0.0000	0.0598	T. stoliczkae
T. tenuis	MOTU-7	0.0039	0.0070	0.0613	T. pseudoscleroptera
T. wuweiensis	MOTU-8	0.0033	0.0050	0.0751	T. obscura
T. sp2	MOTU-9	0.0028	0.0030	0.0264	T. obscura
T. obscura	MOTU-10	0.0101	0.0200	0.0264	T. sp2
T. polyfasciata	MOTU-11	0.0022	0.0030	0.0857	T. bleekeri
T. sp1	MOTU-12	0.0099	0.0100	0.0290	T. papillosolabiatus
T. leptosoma	MOTU-13	0.0022	0.0030	0.0147	T. papillosolabiatus
T. papillosolabiatus	MOTU-14	0.0053	0.0130	0.0147	T. leptosoma
T. sellaefer	MOTU-16	0.0036	0.0070	0.0440	T. minxianensis sp1
T. hsutschouensis	MOTU-18	0.0063	0.0130	0.0320	T. robusta
T. scleroptera	MOTU-6	0.0030	0.0050	0.0040	T. pseudoscleroptera
T. pseudoscleroptera	MOTU-6	0.0040	0.0070	0.0040	T. scleroptera
T. stoliczkae	MOTU-2	0.0060	0.0120	0.0130	T. dalaica
T. dalaica	MOUT-2	0.0040	0.0070	0.0130	T. stoliczkae
T. robusta		0.0310	0.0790
	MOTU-19	0.0082	0.0250	0.0320	T. hsutschouensis
	MOTU-15	0.0077	0.0180	0.0000	T. pappenheimi, T. siluroides
T. minxianensis		0.0160	0.0740	0.0110	T. siluroides
	MOTU-17	0.0016	0.0020	0.0430	T. robusta
	MOTU-15	0.0050	0.0100	0.0000	T. siluroides, T. pappenheimi
T. siluroides	MOTU-15	0.0030	0.0050	0.0000	T. pappenheimi, T. robusta
T. pappenheimi	MOTU-15	0.0020	0.0030	0.0000	T. siluroides, T. minxianensis

The mean and the maximum of intra-group distances, the nearest neighbor (NN), and the minimum distance to the NN for the Nominal species