Fig. 6
From: The evolution of the huntingtin-associated protein 40 (HAP40) in conjunction with huntingtin
Interaction study of zebrafish HAP40 and HTT. Zebrafish HTT was immunoprecipitated with magnetic anti-FLAG beads from cell lysates of DrHTT-HAP40 cells. The DrHTT-HAP40 cells constitutively express zebrafish HAP40 and the expression of zebrafish HTT can be induced by addition of doxycycline. Cell lysates (IP −) and eluates (IP +) were analysed by Western-Blot analysis using anti-Strep (HAP40) or anti-FLAG (HTT) antibodies. Western Blots are representative of three independent experiments