Fig. 5
From: Frequent birth-and-death events throughout perforin-1 evolution
Structure and origin of fish c2PRF1 genes. a domain composition of full-length perforin-1 (human) and c2PRF1 proteins (zebrafish). b amino acid alignment of c2PRF1 proteins (_X) with human perforin-1. Arrows highlight residues probably involved in Ca2+ binding. Hsa, Homo sapiens; Hco, Hippocampus comes; Ame, Astyanax mexicanus; Cse, Cynoglossus semilaevis; Elu, Esox lucius. c Bayesian inference (left) and maximum-parsimony (right) phylogenetic trees from teleost c2PRF1 (red) and PRF1 (black) protein sequences. Node numbers depict posterior probability (left) or robustness as assessed by bootstrapping (right). Dre, Danio rerio; Ame, Astyanax mexicanus; Ssa, Salmo salar; Rbi, Rhinatrema bivittatum; Ola, Oryzias latipes Cel, Caenorhabditis elegans