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Fig. 6 | BMC Evolutionary Biology

Fig. 6

From: The development of early pioneer neurons in the annelid Malacoceros fuliginosus

Fig. 6

Clonal lineage of the cell 2d221 in the embryo of M. fuliginosus. a-t’ Images of fixed, DAPI stained specimens from first to 7th cleavage. The images are labeled either by their stage (1c-64c, 5 h, 6 h) or by the cleavage (cl1-cl7). The positional labels animal and vegetal do not refer to the absolute position in the embryo, but to the relative position of image pairs of the same stage or cleavage. Arrows indicate the spindle direction. u-u′ FISH with probe against Mfu-NeuroD at 6 h stage, posterior part of the embryo. (Yellow asterisks) corresponding landmarks between image pairs, (black asterisks) spindle pole in focus plane, (pb) polar body, (pn) pronucleus. Scale bars: 20 μm

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