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Fig. 4 | BMC Evolutionary Biology

Fig. 4

From: Distant hybrids of Heliocidaris crassispina (♀) and Strongylocentrotus intermedius (♂): identification and mtDNA heteroplasmy analysis

Fig. 4

Amplification of mtDNA in Heliocidaris crassispina (♀) and Strongylocentrotus intermedius (♂) hybrids. a: Mitochondrial genome map for the H. crassispina Fujian population. b: Mitochondrial genome map for the S. intermedius cultured population. c: mtDNA fragment amplification diagram for the H. crassispina (♀) × S. intermedius (♂) hybrids. Primer pair V was selected for detection and identification; the product amplified by primer pair V is boxed in red. HC_F: H. crassispina Fujian population; SI_C: S. intermedius cultured population; HS hybrid: H. crassispina (♀) × S. intermedius (♂) hybrid. d. Electrophoresis gels, showing the results of PCR amplification using primer pairs that were used to amplify mtDNA fragments from the cultured population of S. intermedius. The fragments in the inner ring are more similar to the female parent (H. crassispina), while the fragments in the outer ring are more similar to the male parent (S. intermedius). The PCR products amplified by primer pair V, which was selected for identification, are boxed in red

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