a-c Morphology and infraciliature and of Heterogastrostyla salina nov. spec. from life (a) and after protargol staining (b, c). a Ventral view of a typical individual, arrow marks the contractile vacuole. b, c Ventral (b) and dorsal (c) view of the same individual, arrowheads in (c) depict the caudal cirri. d, e Surroundings of the sampling sites, arrows indicate where the soil samples were collected. f Photograph showing the raw culture in a non-flooded Petri dish. AZM, adoral zone of membranelles; DM, dorsomarginal kinety; FC, frontal cirri; FVC, frontoventral cirri; LMR, left marginal row; Ma, macronuclear nodules; Mi, micronuclei; PVC, postoral ventral cirri; PTVC, pretransverse ventral cirri; RMR, right marginal row; TC, transverse cirri. 1–3, dorsal kineties 1–3. Scale bars = 30 μm (a); 40 μm (b, c).