Fig. 8

Gene expression in trunk EdU⁺ cells. a–e″ 30-min EdU pulse (cyan) at different stages of neurogenesis was combined with FISH (red). Ventral views of the trunk neuroectoderm a, b, c, d, e and transverse sections through the anterior a’, b′, c′, d′, e′, and posterior a″, b″, c″, d″, e″ trunk neuroectoderm are shown. Overlap of EdU⁺ cells with Ct-soxB1a–a″, Ct-ngnb–b″, Ct-ash1c–c″, Ct-neuroDd–d″, and Ct-elav1e–e″ are shown. In panels a–e″, closed arrowheads indicate EdU⁺/gene⁺ cells while open arrowheads show EdU⁻/gene⁺ cells. In ventral view panels a–e, orientation of the animal is indicated in the bottom right corner, and developmental stage is indicated in the top right corner. f–h The proportion of EdU⁺ cells expressing a Ct-soxB1 (F), Ct-ngng, and Ct-ash1h in segments 2–4, 5–7, and 8–10 in the VNC were scored and plotted at different developmental stages. In f–h, capital letters on top of the boxplots (e.g. A, B etc.) indicate statistical significance computed using mixed effects model analysis. The upper and lower bounds of the box plot indicate the 3rd and 1st quartiles while the middle line inside the boxplot indicates the median. The ends of the whiskers represent the 5th and 95th percentiles. Black dots represent outliers (±3 S.D.). In orthogonal views, apical is up, basal is down and yellow dot shows the position of the ventral midline. White dotted line marks the apical boundaries of the neuroectoderm. In ventral views, anterior is to the left. An asterisk denotes the mouth. Ant, anterior; vent, ventral. ST4mid: Stage 4 middle, ST4tt: Stage 4 telotroch, ST5: Stage 5, ST6: Stage 6. Scale bar: 50 μm