Skip to main content
Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Investigating cellular and molecular mechanisms of neurogenesis in Capitella teleta sheds light on the ancestor of Annelida

Fig. 5

Differential EdU labeling associated with the chromatin. a Schematic showing EdU pulse chase experiment with EdU pulse at stage 4 telotroch followed by 3 h of 10 μM thymidine chase and subsequent incubation in ASW until indicated time periods where animals were fixed. (bc) Close-up ventral images showing differential EdU labeling after a 20 h chase. Panels b', c' shows enlarged images of uniform b′ and stippled c′ labeling of EdU+ cells observed at 0 and 20 h post EdU pulse respectively. Arrows indicate probable actively dividing NPCs that exhibit weak EdU labeling due to multiple rounds of mitosis while closed arrowheads indicate uniform EdU labeled cells in the ventrolateral ectoderm. Panels b and c are left and right-side crops of ventral views of the trunk with anterior to left and posterior to the right. Asterisk denotes the mouth. The durations of sea-water incubation following EdU pulse is indicated on the right-hand corners. Scale bar: 50 μm

Back to article page