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Fig. 4 | BMC Evolutionary Biology

Fig. 4

From: Investigating cellular and molecular mechanisms of neurogenesis in Capitella teleta sheds light on the ancestor of Annelida

Fig. 4

Cell-cycle kinetics and dynamics of cell proliferation in the trunk neuroectoderm. a Schematic showing EdU pulse chase experiment with EdU pulse at stage 4 telotroch followed by 3 h of 10 μM thymidine chase and subsequent incubation in sea-water until indicated time periods. (bd) Ventral views b, c, d and orthogonal views (b.1, b.2, c.1, c.2, d.1, d.2) of larval trunk neuroectoderm at three different time intervals (3 h, 12 h and 48 h) shown labeled with EdU (green) and Hoechst (magenta). b.1, c.1 and d.1 indicate orthogonal views along the dashed line labeled “1” and b.2, c.2 and d.2 represent orthogonal views along the dashed line labeled “2” in b, c, d respectively. Arrows in c.1, c.2, d.1, d.2 indicate weakly labeled EdU+ cells which are generally localized on the surface trunk ectoderm while open arrowheads indicate subsurface uniformly labeled EdU+ cells. (ef) Plots showing number of EdU+ cells e, all cells labeled by Hoechst f and proportion of EdU+ relative to Hoechst labeled cells g at different lengths of time after the chase for specific ROIs as indicated in Additional File 7: Fig. S4h. Boxplots are organized into panels by ROI and ordered by time following chase – 0 h (red) and 48 h (blue), with times post EdU pulse indicated in hours on the x-axis. Capital letters above the boxplots indicate statistical groups comparing cell counts or proportions at different times within a ROI. The lowercase letters below the boxplot indicate statistical groups comparing cell counts or proportions for different ROIs at each given chase timing. Different letters represent significant differences with p < 0.05 after correction for multiple comparisons. Panels b, c, d show ventral views with anterior to left and posterior to the right. In all ventral views, asterisk indicates the position of the mouth while the yellow dot in the orthogonal views (b.1, b.2, c.1, c.2, d.1, d.2) indicate the position of the ventral midline. In all orthogonal views, apical is up and basal is down. ST4tt: stage 4 telotroch, ST5e: stage 5 early, ST5mid: stage 5 middle, ST5l: Stage 5 late, ST6e: stage 6 early, ST6mid: stage 6 mid. pt.: prototroch, tt: telotroch. Scale bar: 50 μm

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