Fig. 10

Co-expression of neurogenic homologs in the trunk neuroectoderm. a–c a subset of Ct-soxB1⁺ cells (cyan) which express Ct-ngn (red) along the more ventral regions. d–fCt-ngn (cyan) is expressed over a broad domain in the trunk ectoderm while Ct-ash1 (red) is expressed in a punctate manner throughout the trunk ectoderm overlapping Ct-ngn⁺ cells. g–iCt-neuroD (red) overlaps some Ct-ngn⁺ cells (cyan) in the trunk neuroectoderm although some Ct-neuroD⁺/Ct-ngn⁻ can also be detected. (j–l) Ct-neuroD (cyan) and Ct-ash1 (red) are expressed in non-overlapping domains in the trunk. c', c", f', f", i', i'', l', l'' are orthogonal views through anterior c′,f′, i′, l’ and posterior c″, f″, i″, l” segments of the trunk neuroectoderm shown in c, f, i and l respectively. The asterisk indicates the position of the mouth. c″’, f″’, i″’, l”’ are diagrams representing the orthogonal sections through the trunk neuroectoderm where colored regions represent relative co-expression (gray) or separate expression (cyan/red) of respective genes associated with figure panels c, f, i and l. In all figure panels, closed arrowheads indicate co-expression of two neurogenic homologs in surface cells while open arrowheads indicate co-expression in subsurface cells in respective panels. Arrows indicate non-overlapping expression of neurogenic homologs in panels l’, l”. Orientation of images are indicated on the lower right corner. In all orthogonal views apical is up and the yellow dot denotes the ventral midline. Dashed line indicates the apical edge of the transverse sections. The different developmental stages investigated are indicated at the lower left corner of each figure panel. Vent, ventral. Scale bar: 25 μm