Fig. 2

ACTR8 gene transcript variants in primates using RT-PCR and sequence analysis. a The AluSz6 element-integrated transcript was validated using the V1 and V2 primer pairs. Grey and dotted boxes represent the coding exon and putative exon, respectively. Horizontal arrows indicate the V1 and V2 primer location. b RT-PCR was performed using the tissue cDNA of the crab-eating monkey, and the PCR product was electrophoresed by agarose gel electrophoresis and stained with ethidium bromide. Seven positive bands were observed: 1, cerebellum; 2, cerebrum; 3, heart; 4, kidney; 5, lung; 6, pancreas; 7, spleen; and 8, testis c Seven transcript variants were identified. Grey boxes represent the coding exon and PCR product. Vertical dashed lines represent the 3′(AG) and 5′(GT) splicing sites. The green box indicates the exon 9a, which is longer than exon 9. The orange-colored arrows represent the validation of RT-PCR primers of each transcript variant. d In the crab-eating monkey, RT-PCR was performed using various tissues and specific primer pairs. GAPDH (120 bp) indicates the positive control. e Human, Old world monkey (rhesus monkey, African green monkey), and New world monkey (marmoset, squirrel monkey) cerebellar cDNA were used for the validation of the transcript variant expression