Fig. 3

Fluorescence microscopy images of YFP-VMAT1-expressing HEK293T cells. Cells were transfected with plasmids encoding YFP, YFP-VMAT1, or its variants, pre-treated in the presence or absence of reserpine, and then incubated with FFN206. Cells were imaged by DIC (gray), the fluorescence of YFP (yellow), and FFN206 (blue). The right panels are merged images of YFP and FFN206 fluorescence. Scale bar = 10 μm