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Table 1 Habitat locations, reproductive mode (RM) and molecular identification of Daphnia pulex and Daphnia pulicaria used in this study. All individuals (n) were found to be cyclically parthenogenic (CP) using methods from [58]. Molecular identification based on the mitochondrial (mtDNA) marker NADH dehydrogenase subunit 5 (ND5) allowed us to assign clade membership as in [59]. The nuclear (nDNA) lactate dehydrogenase A (LDH) locus was used to identify individuals that were homozygous for the F allele (lake phenotype) or the S allele (pond phenotype), or were heterozygous for both alleles (hybrid phenotype)

From: The evolution of reproductive isolation in Daphnia

 

mtDNA

nDNA

Location

Clone ID

Lat

Lon

n

RM

ND5 clade

LDH

Ponds

 Center, IL

CEN

40.13

−88.14

3

CP

Panarctic D. pulex

SS

 Dump, IL

DUM

40.24

−87.78

2

CP

Panarctic D. pulex

SS

 Bridge North, IL

BRI

40.12

−87.74

2

CP

Panarctic D. pulex

SS

 Top, IL

TOP

40.24

−87.78

2

CP

Panarctic D. pulex

SS

 Disputed, ON

DIS

42.17

−83.03

3

CP

Panarctic D. pulex

SS

 Solomon, MI

SOL

42.71

−84.38

2

CP

Panarctic D. pulex

SS

 St. Michael, ON

STM

42.23

−83.07

2

CP

Panarctic D. pulex

SS

Lakes

 Clear, IL

CLE

40.14

−87.74

2

CP

Panarctic D. pulex

FF

 Deep, IL

DEE

40.13

−87.74

1

CP

Panarctic D. pulex

FF

 Sportsman’s, IL

SPO

40.14

−87.44

2

CP

Panarctic D. pulex

FF

 Long, IL

LON

40.13

−87.74

1

CP

Panarctic D. pulex

FF

 Hill, MN

HIL

47.01

−93.36

1

CP

Panarctic D. pulex

FF

 Glen, ON

GLE

45.08

−78.30

2

CP

Panarctic D. pulex

FF