Fig. 4

RT-PCR amplification for expression pattern analysis of the BLOC1S2 gene in the crab-eating monkey (a), rhesus monkey (b), and human (c). Original transcript (175 bp), V1 (293 bp), and V2 (239 bp) were amplified using transcript-specific set of primers, Primers 1, 2, and 3, respectively. GAPDH (120 bp) indicates the positive control. In case of V2, see Additional file 1: Figure S6 to check band presence