Fig. 2

Confirmation of ribosomal protein mRNA intron removal by 5′ RACE, and RT-PCR verification of U2 snRNA expression. a 5′ RACE analysis of spliced mRNAs expressed from predicted intron-containing ribosomal protein (RP) genes or b) RT-PCR detection of expression of the candidate S. vortens U2 snRNA. Products have been resolved on 3% agarose gels. Arrows indicate the bands of expected size for cDNA products indicative of intron removal. Inclusion of reverse transcriptase (+) or its omission (−) during the reactions are indicated above each lane. Lanes labeled M contain a molecular weight DNA size ladder with band sizes indicated in base pairs. c Table indicating expected sizes for cDNA products from the RP mRNAs following intron removal based on predicted or EST-confirmed 5′ end, and also for the RT-PCR product for the targeted U2 snRNA segment