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Table 1 Ascorbate peroxidase purification from P. marinus

From: Lacking catalase, a protistan parasite draws on its photosynthetic ancestry to complete an antioxidant repertoire with ascorbate peroxidase

Enrichment Step Total protein
Specific activity
μM min− 1mg− 1
Osmotic lysis 16.1 0.49 100 1.0
DEAE sephacel 2.85 6.63 238a 13.7
Phenyl sepharose 0.033 493.5 206a 1007
  1. aYield of > 100% may reflect the removal of an inhibitor in the DEAE step