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Table 3 Assessment and comparison of genetic diversification levels among the three examined regions (Atlantic (A), western Mediterranean (WM) and eastern Mediterranean (EM)) for Eriphia verrucosa. The computed values for each parameter were obtained after a rarefaction procedure (as described in the Methods section) to the smallest sample size (N) of 42 (from the Atlantic)

From: Phylogeographic and evolutionary history analyses of the warty crab Eriphia verrucosa (Decapoda, Brachyura, Eriphiidae) unveil genetic imprints of a late Pleistocene vicariant event across the Gibraltar Strait, erased by postglacial expansion and admixture among refugial lineages

Genetic diversity indices

Number of replicates (R)

A

(42)

WM

(42)

EM

(42)

Assessment of inter-regional difference (unpaired t-test)

A vs. WM

A vs. EM

WM vs. EM

Haplotype diversity (h)

R = 30

0.808

0.728

0.677

***

***

***

R = 60

0.808

0.728

0.667

***

***

***

Nucleotide diversity (Ï€)

R = 30

0.0039

0.0038

0.0028

**

***

***

R = 60

0.0039

0.0038

0.0027

***

***

***

Mean number of nucleotide differences (K)

R = 30

1.786

1.748

1.272

**

***

***

R = 60

1.786

1.744

1.255

***

***

***

Haplotypic richness (Hapr)

R = 30

13

11.800

12.200

***

*

ns

R = 60

13

11.800

12.216

***

***

ns

Number of private haplotypes (Np)

R = 30

7.500

6.400

4.466

***

***

***

R = 60

7.566

6.550

4.716

***

***

***

Proportion of private haplotypes (Np/N)

R = 30

0.178

0.152

0.106

***

***

***

R = 60

0.180

0.155

0.112

***

***

***

Genetic endemism (Np/Hapr)

R = 30

0.576

0.542

0.361

*

***

***

R = 60

0.582

0.554

0.381

*

***

***

  1. *: Significant difference at P < 0.05; **: Significant difference at P < 0.01; ***: Significant difference at P < 0.001; ns Non-significant difference (P > 0.05). The values, highlighted for each diversity measure, correspond to the mean values obtained for the examined number of replicates. The number of private haplotypes, for each replicate, was determined according to the pattern of distribution of haplotypes in a dataset composed of 126 samples taken randomly from the East Atlantic (42), western Mediterranean (42) and the eastern Mediterranean (42)