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Fig. 2 | BMC Evolutionary Biology

Fig. 2

From: Analysis of the coding sequences of clownfish reveals molecular convergence in the evolution of lifespan

Fig. 2

a Linear depiction of GSTK1 with color coded known functional domains/sites (dimer interface – green, GSH binding – turquoise, sites that serve as both dimer interface and GSH binding – violet, substrate access channel – black) and positively selected sites (in the last common ancestor of the clownfishes with a predicted probability ≥95% – red, in the last common ancestor of the clownfishes with a predicted probability < 95% – orange, in the last common ancestor of Nothobranchius pienaari and Nothobranchius rachovii – blue. b Alignment of GSTK1 orthologs across a wide phylogenetic range of species. Depicted are two protein regions (51–69, 84–89) that contain positively selected sites and functionally relevant sites in close proximity. The color code for positively selected and functionally relevant sites is the same as in panel A. c Clownfish GSTK1 model showing one subunit of the modelled dimer (for an overview see Additional file 3: Figure S1). Selected positions are color coded according to function depicted in the overview scheme at the top. The numbered and colored residue positions (60, 68, 88, 170 and 177) are discussed in detail in the text. Also shown is the GSH substrate (glutathione, light purple) as positioned in the template structure (PDB 1R4W) of the rat GSTK1

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