Table 1 Primers and conditions used for PCR amplification and sequencing of CCL16 from lagomorphs’ gDNA samples
|  | Species amplified | Primers sequence (5′- 3′) | Primer name | Exons amplified | PCR conditions | Fragment length |
|---|---|---|---|---|---|---|
Leporids | Rabbits, hares and cottontails | CTCTCCCTGACACTGCTC | CCL16OrcuF1 | 1 | 95 °C (15 min) 40 cycles: 95 °C (45 s), 56 °C (15 s), 72 °C (10s) 60 °C (10 min) | ~ 250 bp |
GCATAGTTCTGCTTGCAGA | CCL16OrcuR1.3 | |||||
European rabbit, hares, cottontails, volcano rabbit | CAARGAGCRTGATTGACAG | CCL16Orcu F1d | 2 + 3 | 95 °C (15 min) 40 cycles: 95 °C (45 s), 59 °C (20s), 72 °C (45 s) 60 °C (10 min) | ~ 800 bp | |
CCATTAGAAGGCCCAGCC | CCL16OrcuR1e | |||||
Riverine rabbitb | GTTCAGAGGCTGACGGCTC | CCL16OrcuF | ||||
CTGTGCAAATGCAGCCAGC | CCL16OrcuR1b | |||||
Amami rabbitb | GTTCAGAGGCTGACGGCTC | CCL16OrcuF | 98 °C (3 min) 40 cycles: 98 °C (30s), 62 °C (20s), 72 °C (30s) 72 °C (5 min) | ~ 893 bp | ||
CTTGTGGTCTGAGCCAGTGC | CCL16OrcuR1 | |||||
Pygmy rabbitb | GTTCAGAGGCTGACGGCTC | CCL16OrcuF | 95 °C (15 min) 40 cycles: 95 °C (45 s), 59 °C (20s), 72 °C (45 s) 60 °C (10 min) | |||
CTTGTGGTCTGAGCCAGTGC | CCL16OrcuR1 | |||||
|  | All pikas | CATTCACAGTCCTCAGCCC | CCL16OcprF1a | 1 | 95 °C (15 min) 40 cycles: 95 °C (45 s), 60 °C (20s), 72 °C (20s) 60 °C (10 min) | ~ 230 bp |
GGTGGCAGAGAAGTGACAC | CCL16OcprR1 | |||||
American pika, manchurian pika, turuchan pika | CATGTGTGAATCCAGAGGAG | CCL16OcprF2 | 2 + 3 | 95 °C (15 min) 40 cycles: 95 °C (45 s), 58 °C (20s), 72 °C (1 min) 60 °C (10 min) | ~ 930 bp | |
GAGGCAACACAATCACATTG | CCL16OcprR2a | |||||
Palla’s pika, Hoffmann’s pika, steppe pika, Northern pika | GTCAGCCGTCCTTGTTCACC | CCL16OcprF2a | 95 °C (15 min) 40 cycles: 95 °C (45 s), 60 °C (20s), 72 °C (45 s) 60 °C (10 min) | ~ 830 bp | ||
GTAAACCTGCACCAACATAGG | CCL16OcprR2a |