Fig. 4

C. inopinata is sperm-limited. (a) Number of embryos laid in single overnight mating conditions at various temperatures. (b) Number of embryos laid in continuous mating or single overnight mating conditions at 25°C. The “one overnight mating” data in panel (b) is the same from those at 25°C in panel (a). C. inopinata has smaller broods than C. elegans (fog-2) in every condition except 30°C (Wilcoxon rank sum test p<0.0001 for 15 and 20°C; W=349, p=0.004 for 25°C; W=575, p=0.002 for 30°C). However, there is no detectable difference in C. elegans (fog-2) and C. inopinata brood sizes under continuous mating conditions (Wilcoxon rank sum test, W=484, p=0.62). N parental females=38, C. elegans overnight access 15°C; N=28, C. inopinata overnight access 15°C; N=28, C. elegans overnight access 20°C; N=26, C. inopinata overnight access 20°C; N=28, C. elegans overnight access 25°C; N=42, C. inopinata overnight access 25°C; N=28, C. elegans overnight access 30°C; N=28, C. inopinata overnight access 30°C; N=30, C. elegans lifetime access 25°C; N=30, C. inopinata lifetime access 25°C.