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Table 4 Gene-specific primers used for amplification and sequencing Mpc1 gene sequences of barley and wheat

From: Structural and functional divergence of the Mpc1 genes in wheat and barley

Gene

Forward primer (5′ → 3′)

Reverse primer (5′ → 3′)

Annealing temperature. (°C)

Purpose

PCR product length (bp)

DNA/cDNA

HvMpc1-H2

GTAACAGGTGGTCGCTCATT

TTGGAGGAGACGGAGCTG

60

qPCR, gene sequencing

167/167

HvMpc1-H3

GAAGGCAGATGGAACGAAGT

GATGATGGACCACCTGTTG

60

qPCR, gene sequencing

238/177

 

ATGAGGAAGGAAGGAGTGAAGA

TTATAGCGGCATGTCCACAGAG

55

gene sequencing

784/723

 

GGACCGGGACTAATAGGATTTC

GTTTCGTCCTCCTTGCTAGTC

55

promoter sequencing

692/−

 

GGCCTAACGAGCTGAAGTATT

GTTTCGTCCTCCTTGCTAGTC

55

promoter sequencing

319/−

TaMpc1-A2

CCGAACAGACAACGAAATCAAG

CCACCCTGGTGGCAGCT

60

qPCR, gene sequencing

83/83

 

ATGAGGAGGGCGTGCAGT

TTAATCCGCCATGTGCAGGGA

55

gene sequencing

853/729

TaMpc1-B2

CCGAACAGACAACGAAATCAAG

CCGCAGTTAGAGGAAAGCCAT

60

qPCR, gene sequencing

162/162

 

ATGGGGATGAGGACGTGCAG

TTAATACGCCATCTGCAGGGACT

55

gene sequencing

843/738

TaMpc1-D2

CCGAACAGACAACGAAATCAAG

GCGCCCACACCGCGAT

60

qPCR, gene sequencing

218/218

 

ATGGGGAGGAGGGCGTGC

TTAATCTGCCATCTGCAGGGAGT

58

gene sequencing

847/732

TaMpc1-D3

CCGAACAGACAACGAAATCAAG

CAGGTCCAGAGCTAGACAGA

60

qPCR, gene sequencing

161/−

 

ATGGCGACGGAAGGGGTGAAGA

TTAACCCTCTCACTTCGTGCATCC

58

gene sequencing

742/−

TaMpc1-D4

CCGAACAGACAACGAAATCAAG

GCCCCGGAGTTGGAGGA

60

qPCR, gene sequencing

132/132

 

ATGGCGACGGAAGGGGTGAAGA

TTAACCCTCTCACTTCGTGCATCC

60

gene sequencing

791/675