From: Structural and functional divergence of the Mpc1 genes in wheat and barley
Gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | Annealing temperature. (°C) | Purpose | PCR product length (bp) DNA/cDNA |
---|---|---|---|---|---|
HvMpc1-H2 | GTAACAGGTGGTCGCTCATT | TTGGAGGAGACGGAGCTG | 60 | qPCR, gene sequencing | 167/167 |
HvMpc1-H3 | GAAGGCAGATGGAACGAAGT | GATGATGGACCACCTGTTG | 60 | qPCR, gene sequencing | 238/177 |
ATGAGGAAGGAAGGAGTGAAGA | TTATAGCGGCATGTCCACAGAG | 55 | gene sequencing | 784/723 | |
GGACCGGGACTAATAGGATTTC | GTTTCGTCCTCCTTGCTAGTC | 55 | promoter sequencing | 692/− | |
GGCCTAACGAGCTGAAGTATT | GTTTCGTCCTCCTTGCTAGTC | 55 | promoter sequencing | 319/− | |
TaMpc1-A2 | CCGAACAGACAACGAAATCAAG | CCACCCTGGTGGCAGCT | 60 | qPCR, gene sequencing | 83/83 |
ATGAGGAGGGCGTGCAGT | TTAATCCGCCATGTGCAGGGA | 55 | gene sequencing | 853/729 | |
TaMpc1-B2 | CCGAACAGACAACGAAATCAAG | CCGCAGTTAGAGGAAAGCCAT | 60 | qPCR, gene sequencing | 162/162 |
ATGGGGATGAGGACGTGCAG | TTAATACGCCATCTGCAGGGACT | 55 | gene sequencing | 843/738 | |
TaMpc1-D2 | CCGAACAGACAACGAAATCAAG | GCGCCCACACCGCGAT | 60 | qPCR, gene sequencing | 218/218 |
ATGGGGAGGAGGGCGTGC | TTAATCTGCCATCTGCAGGGAGT | 58 | gene sequencing | 847/732 | |
TaMpc1-D3 | CCGAACAGACAACGAAATCAAG | CAGGTCCAGAGCTAGACAGA | 60 | qPCR, gene sequencing | 161/− |
ATGGCGACGGAAGGGGTGAAGA | TTAACCCTCTCACTTCGTGCATCC | 58 | gene sequencing | 742/− | |
TaMpc1-D4 | CCGAACAGACAACGAAATCAAG | GCCCCGGAGTTGGAGGA | 60 | qPCR, gene sequencing | 132/132 |
ATGGCGACGGAAGGGGTGAAGA | TTAACCCTCTCACTTCGTGCATCC | 60 | gene sequencing | 791/675 |