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Table 4 List of strains used for PCR amplification. Number of repeats and repeat motifs in Sgs3 and Sgs4 in populations of D. melanogaster and D. mauritiana. Sequences of Sgs4 for Oregon R and Samarkand strains are from [83]. * indicate lines also used in the Drosophila Nexus project. @ indicate suspected artifactual repeat losses during cloning. PTC indicates the presence of a premature termination codon

From: Evolution of salivary glue genes in Drosophila species

protein species sample Origin nr of repeats type of repeat remarks
Sgs3 D. melanogaster Cayenne French Guyana 29 (K/N)(P/Q/A)TTT  
   Chavroche France 29 (K/N)(P/Q/A)TTT  
   Chavroche2 France 29 (K/N)(P/Q/A)TTT  
   Chavroche3 France 30 (K/N)(P/Q/A)TTT  
   Cotonou Benin 31 (K/N)(P/Q/A)TTT  
   Delhi1 India 27 (K/N)(P/Q/A)TTT  
   Delhi2 India 29 (K/N)(P/Q/A)TTT  
   Delhi B India 27 (K/N)(P/Q/A)TTT  
   Gally A France 29 (K/N)(P/Q/A)TTT  
   Gally B France 29 (K/N)(P/Q/A)TTT  
   Gally C France 29 (K/N)(P/Q/A)TTT  
   Gally D France 29 (K/N)(P/Q/A)TTT  
   EF1 B Ethiopia* 24 (K/N)(P/Q/A)TTT  
   EF1 3 Ethiopia* 29 (K/N)(P/Q/A)TTT  
   EG15N Cairo, Egypt* 30 (K/N)(P/Q/A)TTT  
   EG16N Cairo, Egypt* > 25 (K/N)(P/Q/A)TTT  
   EG25N Cairo, Egypt* 29 (K/N)(P/Q/A)TTT  
   EG28N Cairo, Egypt* > 29 (K/N)(P/Q/A)TTT  
   EG33N a Cairo, Egypt* 12@ (K/N)(P/Q/A)TTT  
   EG33N c Cairo, Egypt* 31 (K/N)(P/Q/A)TTT  
   EG34N Cairo, Egypt* 7@ (K/N)(P/Q/A)TTT  
   EG55N Cairo, Egypt* 23 (K/N)(P/Q/A)TTT  
   EG59N Cairo, Egypt* 22 (K/N)(P/Q/A)TTT  
   EG74N Cairo, Egypt* 23 (K/N)(P/Q/A)TTT  
  D. mauritiana GM21 Grande Montagne (Rodrigues Island) 5 CAPPTRPP(T)n  
   GM23a Grande Montagne (Rodrigues Island) 5 CAPPTRPP(T)n  
   GM23b Grande Montagne (Rodrigues Island) 3 CAPPTRPP(T)n  
   GM24 Grande Montagne (Rodrigues Island) 4 CAPPTRPP(T)n  
   GM25 Grande Montagne (Rodrigues Island) 5 CAPPTRPP(T)n  
   GRNM1 Gorges de la Rivière Noire (Mauritius) 5 CAPPTRPP(T)n  
   MaurII-704 Mauritius 5 CAPPTRPP(T)n  
   MaurII-a Mauritius 5 CAPPTRPP(T)n  
Sgs4 D. melanogaster CG12181 reference strain Iso1 20 C(K/R/E)TEPP(R/T)  
   OregonR lab strain (from [83]) 22 C(K/R/E)TEPP(R/T)  
   Samarkand [83] 21 C(K/R/E)TEPP(R/T)  
   Canton S Lab strain > 21 C(K/R/E)TEPP(R/T)  
   Cayenne1 French Guyana > 21 C(K/R/E)TEPP(R/T)  
   Cayenne2 French Guyana > 22 C(K/R/E)TEPP(R/T)  
   Cayenne3 French Guyana > 21 C(K/R/E)TEPP(R/T)  
   Chavroche1 France > 22 C(K/R/E)TEPP(R/T)  
   Chavroche3 France > 22 C(K/R/E)TEPP(R/T)  
   Comores1 Comores > 22 C(K/R/E)TEPP(R/T)  
   Comores2 Comores > 22 C(K/R/E)TEPP(R/T)  
   Cotonou Benin > 22 C(K/R/E)TEPP(R/T)  
   Delhi1 India > 21 C(K/R/E)TEPP(R/T)  
   Delhi2 India > 21 C(K/R/E)TEPP(R/T)  
   Gally1 France > 20 C(K/R/E)TEPP(R/T)  
   Gally2 France > 20 C(K/R/E)TEPP(R/T)  
   EF1 Ethiopia* > 22 C(K/R/E)TEPP(R/T)  
   Tai1 Ivory Coast > 20 C(K/R/E)TEPP(R/T)  
   Tai2 Ivory Coast > 20 C(K/R/E)TEPP(R/T)  
   EG15N Cairo, Egypt* > 26 C(K/R/E)TEPP(R/T) PTC
   EG16N Cairo, Egypt* 22 C(K/R/E)TEPP(R/T) PTC
   EG25N Cairo, Egypt* 20 C(K/R/E)TEPP(R/T) PTC
   EG28N Cairo, Egypt* 20 C(K/R/E)TEPP(R/T) PTC
   EG33N Cairo, Egypt* 20 C(K/R/E)TEPP(R/T) PTC
   EG34N Cairo, Egypt* 22 C(K/R/E)TEPP(R/T) PTC
   EG36N Cairo, Egypt* 22 C(K/R/E)TEPP(R/T) PTC
   EG44N Cairo, Egypt* > 26 C(K/R/E)TEPP(R/T) PTC
   EG55N Cairo, Egypt* > 26 C(K/R/E)TEPP(R/T) PTC
   EG59N Cairo, Egypt* > 26 C(K/R/E)TEPP(R/T) PTC
   EG74N Cairo, Egypt* > 26 C(K/R/E)TEPP(R/T)  
   ZI395 Zambia* 25 C(K/R/E)TEPP(R/T)  
   ZI420 Zambia* 18 C(K/R/E)TEPP(R/T)  
  D.mauritiana GM22 Grande Montagne (Rodrigues Island) > 30 C(N/D)TEPP  
   GM23 Grande Montagne (Rodrigues Island) > 31 C(N/D)TEPP  
   GM25 Grande Montagne (Rodrigues Island) > 30 C(N/D)TEPP  
   GRNM1 Gorges de la Rivière Noire (Mauritius) > 27 C(N/D)TEPP  
   GRNM2 Gorges de la Rivière Noire (Mauritius) > 32 C(N/D)TEPP  
   GRNM3 Gorges de la Rivière Noire (Mauritius) > 27 C(N/D)TEPP  
   GRNM6 Gorges de la Rivière Noire (Mauritius) > 24 C(N/D)TEPP  
   MaurII-a Mauritius > 28 C(N/D)TEPP  
   MaurII-704 Mauritius > 28 C(N/D)TEPP  
Sequence checking D. sechellia   Praslin Island    
  D. santomea STO3 Sao Tomé    
  D. virilis   Spain    
  D. biarmipes   India