Table 4 List of strains used for PCR amplification. Number of repeats and repeat motifs in Sgs3 and Sgs4 in populations of D. melanogaster and D. mauritiana. Sequences of Sgs4 for Oregon R and Samarkand strains are from [83]. * indicate lines also used in the Drosophila Nexus project. @ indicate suspected artifactual repeat losses during cloning. PTC indicates the presence of a premature termination codon
From: Evolution of salivary glue genes in Drosophila species
protein | species | sample | Origin | nr of repeats | type of repeat | remarks |
|---|---|---|---|---|---|---|
Sgs3 | D. melanogaster | Cayenne | French Guyana | 29 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | Chavroche | France | 29 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | Chavroche2 | France | 29 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | Chavroche3 | France | 30 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | Cotonou | Benin | 31 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | Delhi1 | India | 27 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | Delhi2 | India | 29 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | Delhi B | India | 27 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | Gally A | France | 29 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | Gally B | France | 29 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | Gally C | France | 29 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | Gally D | France | 29 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | EF1 B | Ethiopia* | 24 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | EF1 3 | Ethiopia* | 29 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | EG15N | Cairo, Egypt* | 30 | (K/N)(P/Q/A)TTT | Â |
|  |  | EG16N | Cairo, Egypt* | > 25 | (K/N)(P/Q/A)TTT |  |
| Â | Â | EG25N | Cairo, Egypt* | 29 | (K/N)(P/Q/A)TTT | Â |
|  |  | EG28N | Cairo, Egypt* | > 29 | (K/N)(P/Q/A)TTT |  |
| Â | Â | EG33N a | Cairo, Egypt* | 12@ | (K/N)(P/Q/A)TTT | Â |
| Â | Â | EG33N c | Cairo, Egypt* | 31 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | EG34N | Cairo, Egypt* | 7@ | (K/N)(P/Q/A)TTT | Â |
| Â | Â | EG55N | Cairo, Egypt* | 23 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | EG59N | Cairo, Egypt* | 22 | (K/N)(P/Q/A)TTT | Â |
| Â | Â | EG74N | Cairo, Egypt* | 23 | (K/N)(P/Q/A)TTT | Â |
| Â | D. mauritiana | GM21 | Grande Montagne (Rodrigues Island) | 5 | CAPPTRPP(T)n | Â |
| Â | Â | GM23a | Grande Montagne (Rodrigues Island) | 5 | CAPPTRPP(T)n | Â |
| Â | Â | GM23b | Grande Montagne (Rodrigues Island) | 3 | CAPPTRPP(T)n | Â |
| Â | Â | GM24 | Grande Montagne (Rodrigues Island) | 4 | CAPPTRPP(T)n | Â |
| Â | Â | GM25 | Grande Montagne (Rodrigues Island) | 5 | CAPPTRPP(T)n | Â |
|  |  | GRNM1 | Gorges de la Rivière Noire (Mauritius) | 5 | CAPPTRPP(T)n |  |
| Â | Â | MaurII-704 | Mauritius | 5 | CAPPTRPP(T)n | Â |
| Â | Â | MaurII-a | Mauritius | 5 | CAPPTRPP(T)n | Â |
Sgs4 | D. melanogaster | CG12181 | reference strain Iso1 | 20 | C(K/R/E)TEPP(R/T) | Â |
| Â | Â | OregonR | lab strain (from [83]) | 22 | C(K/R/E)TEPP(R/T) | Â |
| Â | Â | Samarkand | [83] | 21 | C(K/R/E)TEPP(R/T) | Â |
|  |  | Canton S | Lab strain | > 21 | C(K/R/E)TEPP(R/T) |  |
|  |  | Cayenne1 | French Guyana | > 21 | C(K/R/E)TEPP(R/T) |  |
|  |  | Cayenne2 | French Guyana | > 22 | C(K/R/E)TEPP(R/T) |  |
|  |  | Cayenne3 | French Guyana | > 21 | C(K/R/E)TEPP(R/T) |  |
|  |  | Chavroche1 | France | > 22 | C(K/R/E)TEPP(R/T) |  |
|  |  | Chavroche3 | France | > 22 | C(K/R/E)TEPP(R/T) |  |
|  |  | Comores1 | Comores | > 22 | C(K/R/E)TEPP(R/T) |  |
|  |  | Comores2 | Comores | > 22 | C(K/R/E)TEPP(R/T) |  |
|  |  | Cotonou | Benin | > 22 | C(K/R/E)TEPP(R/T) |  |
|  |  | Delhi1 | India | > 21 | C(K/R/E)TEPP(R/T) |  |
|  |  | Delhi2 | India | > 21 | C(K/R/E)TEPP(R/T) |  |
|  |  | Gally1 | France | > 20 | C(K/R/E)TEPP(R/T) |  |
|  |  | Gally2 | France | > 20 | C(K/R/E)TEPP(R/T) |  |
|  |  | EF1 | Ethiopia* | > 22 | C(K/R/E)TEPP(R/T) |  |
|  |  | Tai1 | Ivory Coast | > 20 | C(K/R/E)TEPP(R/T) |  |
|  |  | Tai2 | Ivory Coast | > 20 | C(K/R/E)TEPP(R/T) |  |
|  |  | EG15N | Cairo, Egypt* | > 26 | C(K/R/E)TEPP(R/T) | PTC |
| Â | Â | EG16N | Cairo, Egypt* | 22 | C(K/R/E)TEPP(R/T) | PTC |
| Â | Â | EG25N | Cairo, Egypt* | 20 | C(K/R/E)TEPP(R/T) | PTC |
| Â | Â | EG28N | Cairo, Egypt* | 20 | C(K/R/E)TEPP(R/T) | PTC |
| Â | Â | EG33N | Cairo, Egypt* | 20 | C(K/R/E)TEPP(R/T) | PTC |
| Â | Â | EG34N | Cairo, Egypt* | 22 | C(K/R/E)TEPP(R/T) | PTC |
| Â | Â | EG36N | Cairo, Egypt* | 22 | C(K/R/E)TEPP(R/T) | PTC |
|  |  | EG44N | Cairo, Egypt* | > 26 | C(K/R/E)TEPP(R/T) | PTC |
|  |  | EG55N | Cairo, Egypt* | > 26 | C(K/R/E)TEPP(R/T) | PTC |
|  |  | EG59N | Cairo, Egypt* | > 26 | C(K/R/E)TEPP(R/T) | PTC |
|  |  | EG74N | Cairo, Egypt* | > 26 | C(K/R/E)TEPP(R/T) |  |
| Â | Â | ZI395 | Zambia* | 25 | C(K/R/E)TEPP(R/T) | Â |
| Â | Â | ZI420 | Zambia* | 18 | C(K/R/E)TEPP(R/T) | Â |
|  | D.mauritiana | GM22 | Grande Montagne (Rodrigues Island) | > 30 | C(N/D)TEPP |  |
|  |  | GM23 | Grande Montagne (Rodrigues Island) | > 31 | C(N/D)TEPP |  |
|  |  | GM25 | Grande Montagne (Rodrigues Island) | > 30 | C(N/D)TEPP |  |
|  |  | GRNM1 | Gorges de la Rivière Noire (Mauritius) | > 27 | C(N/D)TEPP |  |
|  |  | GRNM2 | Gorges de la Rivière Noire (Mauritius) | > 32 | C(N/D)TEPP |  |
|  |  | GRNM3 | Gorges de la Rivière Noire (Mauritius) | > 27 | C(N/D)TEPP |  |
|  |  | GRNM6 | Gorges de la Rivière Noire (Mauritius) | > 24 | C(N/D)TEPP |  |
|  |  | MaurII-a | Mauritius | > 28 | C(N/D)TEPP |  |
|  |  | MaurII-704 | Mauritius | > 28 | C(N/D)TEPP |  |
Sequence checking | D. sechellia | Â | Praslin Island | Â | Â | Â |
|  | D. santomea | STO3 | Sao Tomé |  |  |  |
| Â | D. virilis | Â | Spain | Â | Â | Â |
| Â | D. biarmipes | Â | India | Â | Â | Â |