Fig. 6

Expression profile of ogn1 and ogn2 in gilthead sea bream bone-derived primary MSC cultures in osteogenic conditions. (a) Representative images of gilthead sea bream cells derived from vertebra growing in control (GM) or osteogenic (OM) conditions at days 5 and 20 of culture development. The arrowheads indicate nodules of mineralization. The images were acquired using a 20x magnification and a scale bar is also indicated in the image (100 μm). Normalized expression of (b) op; (c) ogn1 and (d) ogn2 in bone-derived cells growing in GM or OM at different days of the culture (5 to 20). Results are shown as the mean ± sem (N = 5–8 independent cultures). Normalized expression (copy number) was determined using the geometric mean of the reference genes ef1α and rps18. A two-way ANOVA followed by a Fisher’s Least Significant Difference (LSD) post-test was performed to identify differences in gene expression among the experimental groups. Significant differences (p < 0.05) between culture conditions are represented with an asterisk and significant differences in each culture condition across time are represented with letters (different letters denote significant differences, p < 0.05)