Fig. 3

Transitions of fertilization types during speciation. Transitions and ancestral reconstruction of fertilization types for 44 cichlids from Lake Tanganyika were shown. a) Ancestral states of nodes (1–13) were shown and statistical tests were carried out in terms of likelihood ratios (2ΔlnL) (Additional file 7: Table S2). Asterisks indicate that ancestral states of the fertilization are statistically dominant (Additional file 7: Table S2). Fertilization types, the glycosylation state of SPP120, and the expression of SPP120 in relation to β-actin (N = 3 each species) are also shown. b) Transition rates and ancestral states were calculated in BayesTraits version 2.0. Arrows indicate transition rates (Oso, Qsb, Qbo), which are an indicator of how likely a particular transition is to have occurred. Qso indicates rates from substrate fertilization to oral fertilization, Qsb is from substrate to stocked sperm fertilization, and Qbo is from stocked-sperm fertilization to oral fertilization. Histograms of each transition rate are also shown