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prs1
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prs2
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prs3
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prs4
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prs5
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prs6
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prs7
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prs8
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prs9
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prs10
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prs11
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prs12
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prs13
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prs14
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prs15
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prs16
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prs17
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prs18
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Cr-BSA | 0.65 b | 0.39 b | 0.49 b | N/D | 0.52 ab | 1.33 a | N/D | N/D | 0.69 a | 0.53 a | 0.50 ab | N/D | N/D | N/D | 0.55 b | 0.46 a | 0.75 a | 0.71 a |
Cr-collagen | 0.03 c | 0.04 c | 0.03 c | N/D | 0.03 b | 0.15 b | N/D | N/D | 0.03 b | N/D | 0.12 b | N/D | 0.01 b | N/D | 0.16 c | 1.01 a | 0.03 b | 0.05 b |
Cr-milk | 1.00 a | 1.01 a | 1.01 a | D | 1.02 a | 1.06 a | N/D | D | 1.00 a | 1.03 a | 1.09 a | D | 1.08 a | D | 1.02 a | 1.02 a | 1.08 a | 1.01 a |
Cr-waterb | 0.03 c | 0.03 c | 0.04 c | N/D | N/D | 0.75 ab | D | N/D | 0.04 b | N/D | 0.37 b | N/D | N/D | N/D | 0.23 bc | 0.93 a | 0.04 b | 0.05 b |
- N/D no detectable expression, D detectable expression
- aGene expression of S8A serine protease genes was determined by RT-qPCR during growth of C. rosea on bovine serum albumin (Cr-BSA), collagen (Cr-collagen), milk powder (Cr-milk) or in water (Cr-water). Relative expression is calculated as the ratio between the target gene and tubulin using the 2–ΔΔCT method. Different letters indicate significant differences (P ≤ 0.05) between treatments for each gene as determined by the Fisher’s least significant difference (LSD) test. The statistical analysis was performed on a minimum of three biological replicates
- bOne biological replicate in the Cr-water treatment deviated substantially from the other four replicates and was considered as an outlier, and hence excluded from the analysis