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Table 4 Expression analysisa of selected Clonostachys rosea S8A serine protease genes during growth on different protein sources

From: Comparative evolutionary histories of fungal proteases reveal gene gains in the mycoparasitic and nematode-parasitic fungus Clonostachys rosea

 

prs1

prs2

prs3

prs4

prs5

prs6

prs7

prs8

prs9

prs10

prs11

prs12

prs13

prs14

prs15

prs16

prs17

prs18

Cr-BSA

0.65 b

0.39 b

0.49 b

N/D

0.52 ab

1.33 a

N/D

N/D

0.69 a

0.53 a

0.50 ab

N/D

N/D

N/D

0.55 b

0.46 a

0.75 a

0.71 a

Cr-collagen

0.03 c

0.04 c

0.03 c

N/D

0.03 b

0.15 b

N/D

N/D

0.03 b

N/D

0.12 b

N/D

0.01 b

N/D

0.16 c

1.01 a

0.03 b

0.05 b

Cr-milk

1.00 a

1.01 a

1.01 a

D

1.02 a

1.06 a

N/D

D

1.00 a

1.03 a

1.09 a

D

1.08 a

D

1.02 a

1.02 a

1.08 a

1.01 a

Cr-waterb

0.03 c

0.03 c

0.04 c

N/D

N/D

0.75 ab

D

N/D

0.04 b

N/D

0.37 b

N/D

N/D

N/D

0.23 bc

0.93 a

0.04 b

0.05 b

  1. N/D no detectable expression, D detectable expression
  2. aGene expression of S8A serine protease genes was determined by RT-qPCR during growth of C. rosea on bovine serum albumin (Cr-BSA), collagen (Cr-collagen), milk powder (Cr-milk) or in water (Cr-water). Relative expression is calculated as the ratio between the target gene and tubulin using the 2–ΔΔCT method. Different letters indicate significant differences (P ≤ 0.05) between treatments for each gene as determined by the Fisher’s least significant difference (LSD) test. The statistical analysis was performed on a minimum of three biological replicates
  3. bOne biological replicate in the Cr-water treatment deviated substantially from the other four replicates and was considered as an outlier, and hence excluded from the analysis