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Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Comparative evolutionary histories of fungal proteases reveal gene gains in the mycoparasitic and nematode-parasitic fungus Clonostachys rosea

Fig. 5

Reverse conservation analysis of two groups of S08.054 serine proteases. Amino acid conservation was estimated using Rate4Site, based on a Clustal W alignment of clade I (solid line) and clade Ib (dashed line) serine proteases (Fig. 2) and plotted as W mean. The y-axis represents arbitrary units while a horizontal line indicates a 0.5 standard deviation cut-off (I). The x-axis represents residue position in reference to C. rosea BN869_T00009071 (PRS16), asterisks (*) indicate the positions of predicted active site residues (147, 178, 242, 243, 270, 330, 333), diamonds (♦) indicates predicted catalytic residue positions (147, 178, 333), CB-I and CB-II indicates the positions of the predicted calcium binding-I (284, 286, 309) and calcium binding-II (118, 121, 122) sites, respectively, boxed P indicate residues evolving under strong (Bayes factor ≥ 50) positive selection (62, 72, 231, 328), P indicate residues evolving under weak (Bayes factor 10–49) positive selection (70, 165, 172, 207, 236, 245, 377). Regions that display signs of functional divergence, high variation (I ≥ 0.5) in one group in combination with low variation (I < 0.5) in the other group, are labelled I through VII

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