Fig. 2

Phylogenetic relationship of S8A serine proteases among fungal species. Amino acid sequences of S8A serine proteases were aligned by Clustal W and used to construct a phylogenetic tree using the maximum likelihood methods in MEGA ver. 6. Branch support values (bootstrap proportions ≥50%) are associated with nodes. Phylogenetic groups referred to as clade I and clade Ib are indicated, as are the S08.005 subtilisin and S08.054 proteinase K groups. Abbreviations: CR = Clonostachys rosea, FG = Fusarium graminearum, FS = F. solani, HM = Hirsutella minnesotensis, HT = H. thompsonii, HS = H. sinensis, TR = Trichoderma reesei, TV = T. virens, TA = T. atroviride and NC = Neurospora crassa