Table 4 Spatial Analysis of Molecular Variance (SAMOVA) of the Pantosteus plebeius-nebuliferus species-group baes on cytb|GHI. Only the results for K = 2, 4, and 8 are shown, as they are meaningful in phylogenetic, systematic, and biogeographic terms

One genetic pool (via AMOVA)

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0.9028*|0.6904*

Pantosteus plebeius/Pantosteus nebuliferus

2

(Palomas/Casas Grandes/Santa Maria/Del Carmen/Conchos/Fuerte/Mezquital) (Piaxtla/Nazas)

0.3545*|0.1707

0.8742*|0.6606*

0.9188*|0.7185*

Phylogenetic inference (Clades)

4

(I) (II) (III) (IV)

0.7685*|0.6166*

0.6324*|0.2715*

0.9149*|0.7207*

Best genetic structure identified without geographic information

8

(Pamolas) (Casas Grandes) (Santa Maria) (Del Carmen) (Conchos/Fuerte) (Piaxtla) (Nazas) (Mezquital)

0.8784*|0.7460*

0.2051*|0.2156

0.9033*|0.6912*