Table 3 Oligonucleotide primer sequences and the conditions used for PCR amplification of genes amplified from Eurhoptus samples
Gene | Forward Primer | Reverse Primer | Anneal temp | Cycles | Reference |
|---|---|---|---|---|---|
Cytochrome oxidase I | CAACATTTATTTTGATTTTTTGG (‘Jerry’) | TCCAATGCACTAATCTGCCATATTA (‘Pat’) | 50 | 35 | Simon et al., 1994 |
CAD | AGCACGAAAATHGGNAGYTCNATGAARAG (CD821F) | GCTATGTTGTTNGGNAGYTGDCCNCCCAT (CD1098R2) | 53 | 37 | Wild & Maddison 2008 |
kkv | TCGACCATHGCCAAYATCATGGA (KKV2768F) | GTACCNACDATNACNGCCATCAT (KKV3023R) | 48 | 40 | Polihronakis & Caterino, 2012 |
ITS2 | GTTTCCGTAGGTGAACCTGC (TW81) | AATGTGCGTTCGAAATGTCG (HITR) | 50 | 35 | Richards et al., 1997 |