Phylogeography of freshwater planorbid snails reveals diversification patterns in Eurasian continental islands

Table 2 Information on primers and PCR conditions used in this study

Primer	Direction	Sequence5’-3’	PCR condition	Reference
CO1
LCO1490	Forward	GGTCAACAATCATAAAGATATTGG	94 °C 4 min, (94 °C 30 s, 48 °C 30 s, 72 °C 90 s) × 34, 72 °C 2 min	Folmer et al. [110], PCR condition was slightly modified.
HCO2198	Reverse	TAAACTTCAGGGTGACCAAAAAATCA
16S
16Sar-L	Forward	CGCCTGTTTATCAAAAACAT	94 °C 4 min, (94 °C 30 s, 40 °C 30 s, 72 °C 60 s) × 34, 72 °C 5 min	Palmubi et al. [111], PCR condition was slightly modified.
16Sbr-H	Reverse	CCGGTCTGAACTCAGATCACGT
H3
H3PulF	Forward	GGAGGCAAGGCCCCACGTAARCA	94 °C 3 min, (94 °C 15 s, 57 °C 30 s, 72 °C 40 s) × 40, 72 °C 1 min	Uit de Weerd and Gittenberger [112].
H3PulR	Reverse	TTGGCGTGGATGGCGCACARG

ISSN: 2730-7182