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Fig. 1 | BMC Evolutionary Biology

Fig. 1

From: Hampered motility promotes the evolution of wrinkly phenotype in Bacillus subtilis

Fig. 1

WS morphotypes exhibit elevated wrinkle formation and appear especially in non-motile mutant. a Microscopy images of WS morphotypes isolated from WT and Δhag strains grown on LB medium. Representative images for the phenotype of each detected mutation, indicated in parentheses, are displayed. The scale bar represents 2 mm. b Relative frequency of WS morphotypes in the pellicle biofilms of various B. subtilis derivatives grown on MSgg medium. Y-axis shows the frequency of WS colony types (1 equals to 100% of the colonies being wrinkly). Boxes represent quartile 1–3, the line represents the median and whiskers indicate the upper and lower inner fence and dots represent outliers. Asterisks indicate significant differences (two sample Student’s t test assuming unequal variances: P values: Δhag, 8.27∙10− 6; ΔflgE, 0.013; ΔfliF, 1.23∙10− 7; ΔsigD, 2.21∙10− 5, ΔmotA, 0.0125; n ≥ 9). Raw data available in Additional file 1: Supplementary Dataset 1. c Schematic representation of the SinR protein with domains and location of the detected mutations depicted

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