Fig. 8

qPCR analysis for genes glnG and amtB in mutant adapted to nitrogen limitation: Gene expression analysis for genes glnG and amtB for wild-type ancestral strain and mutant with glnG V18L mutation under nutrient limiting conditions of nitrogen limitation and magnesium limitation. Increased expression levels for both the genes were seen only under limiting nitrogen conditions. Relative expression levels with respect to wild-type (horizontal line) plotted