Skip to main content
Fig. 3 | BMC Evolutionary Biology

Fig. 3

From: A targeted in situ hybridization screen identifies putative seminal fluid proteins in a simultaneously hermaphroditic flatworm

Fig. 3

Summary of the expression patterns (visually identified by ISH) for all 146 tail-specific transcripts investigated and secretion predictions for those 76 with prostate-limited expression. In (a) the transcripts are listed in descending order of their fold-change (= solid vertical bar) observed in an earlier study comparing transcript expression in octets (groups of eight worms) compared to isolated worms (Ramm et al: Sex Allocation Plasticity on a Transcriptome Scale: Socially-Sensitive Gene Expression in the Hermaphroditic Flatworm Macrostomum lignano, submitted). Note that the 10 transcripts at the end of the list have fold-change values less than one (i.e. they had lower expression in octets than in isolated worms). For each transcript, we document its site of expression, indicated by the colour of the bar (green: prostate-limited; blue: prostate + elsewhere; orange: not prostate; grey: unknown) and by the abbreviated tissue assignments (pr: prostate; he: head; te: testis; ov: ovary; go: gonad; mi: unspecific in middle section; ph: pharynx; pg: pharyngeal glands; gu: gut; eg: egg; rh: rhabdites; an: antrum; cg: cement glands; st: stylet; un: unspecific; ad: adhesive glands). In addition, for each of the 76 prostate-limited transcripts we assign a unique number (Mlig-pro1–76, see main text for explanation) and sought bioinformatic evidence for secretion. For the 50 transcripts for which we could perform bioinformatic analyses, we indicate positive evidence for secretion based on predictions from SignalP (black circle: signal peptide present); SecretomeP (black circle: non-classically secreted); ProtComp (black circle: extra-cellularly secreted or membrane-bound extracellularly secreted; grey circle: plasma membrane); and TMHMM (black circle: outside the cell; grey circle: trans-membrane helix). Note that for 11 transcripts, it was not possible to identify the open reading frame and bioinformatics analyses could therefore not be performed (denoted ‘n/a’). The results from each of these analyses are summarized in panels (b-e)

Back to article page