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Table 4 Analysis of molecular variance assessing population genetic structure and testing for partitioning of Carcinus aestuarii genetic variation under alternative biogeographic hypotheses

From: Parapatric genetic divergence among deep evolutionary lineages in the Mediterranean green crab, Carcinus aestuarii (Brachyura, Portunoidea, Carcinidae), accounts for a sharp phylogeographic break in the Eastern Mediterranean

Hypothesis tested Nucleotide divergence (Tajima and Nei distance) Haplotype frequency
1-Based on the geographic origin of examined populations (separation across the Siculo-Tunisian Strait):
Western Mediterranean vs. Eastern Mediterranean ΦSC = 0.588***
ΦST = 0.655***
ΦCT = 0.164*
FSC = 0.080***
FST = 0.156***
FCT = 0.082**
2-Based on the geographic origin of examined populations (separation among the different defined geographic regions):
Algerian Basin vs. Afro-Sicilian Basin vs. Adriatic Sea vs. Ionian Sea vs. Aegean Sea vs. Sea of Marmara ΦSC = 0.143***
ΦST = 0.656***
ΦCT = 0.598***
FSC = 0.045***
FST = 0.121***
FCT = 0.079***
3-Based on the outcome of TCS haplotype network:
(Tabarka + Bizerte + Sidi Rais + Kelibia + Benikhiar + Venice + Pomer + Amvrakikos + Lefkada + Navarino) vs. (Monastir + Chebba + Sfax + Djerba + Tripoli + Mosrata) vs. (Alexandroupolis + Izmir Bay + Enez Dalyan Lagoon + Dardanelles Strait + Prince’s Islands + Bosphorus Strait) ΦSC = 0.087***
ΦST = 0.701***
ΦCT = 0.673***
FSC = 0.018***
FST = 0.148***
FCT = 0.132***
4-Based on the outcome of genetic differentiation and MDS plot:
(Tabarka + Bizerte + Sidi Rais + Kelibia + Benikhiar + Monastir + Chebba + Sfax + Djerba + Tripoli + Mosrata + Venice + Pomer + Amvrakikos + Lefkada + Navarino) vs. (Alexandroupolis + Izmir Bay + Enez Dalyan Lagoon + Dardanelles Strait + Prince’s Islands + Bosphorus Strait) ΦSC = 0.167***
ΦST = 0.795***
ΦCT = 0.754***
FSC = 0.045***
FST = 0.197***
FCT = 0.159***
  1. *: P < 0.05; **: P < 0.01; ***: P < 0.001. The description of populations’ attributions to basins and regions is reported in Table 1. The second grouping scheme corresponds to sub-basins nearly matching marine ecoregions identified by Spalding [92] for the Mediterranean, namely the Adriatic, Ionian and Aegean seas. In particular, we designated the Western Mediterranean marine ecoregion (in Spalding delineation of Mediterranean marine ecoregion) as the Algerian Basin, and re-named the Tunisian Plateau/Gulf of Sidra (as identified in Spalding [92]) as the Afro-Sicilian Basin. We also considered the Black Sea ecoregion (delineated in Spalding [92]) as the Marmara Sea in our grouping scheme